Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.)

BMC Genetics
Jiangping BaiJinlin Zhang

Abstract

The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L.) cultivar 'Longshu 3', with similar characteristics to SnRK2 from other plant species in gene structure, motif distribution and secondary structures. The C-terminal regions were highly divergent among StSnRK2s, while they all carried the similar Ser/Thr protein kinase domain. The fluorescence of GFP fused with StSnRK2.1, StSnRK2.2, StSnRK2.6, StSnRK2.7 and StSnRK2.8 was detected in the nucleus and cytoplasm of onion epidermal cells with StSnRK2.3 and StSnRK2.4 mainly associated to the nucleus while StSnRK2.5 to subcellular organelles. Expression level analysis by qRT-PCR showed that StSnRK2.1, 2.2, 2.5 and 2.6 were more than 1 fold higher in the root than in the leaf, tuber and stem tissues. The expressions of StSnRK2.3, 2.7, and...Continue Reading

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Citations

Apr 30, 2020·Antioxidants·Isabella RomeoTiziana Marino
Feb 20, 2019·International Journal of Molecular Sciences·Xiaohui YangLiping Jin
Feb 3, 2020·Molecular Genetics and Genomics : MGG·Zhangxiong LiuLijuan Qiu
Jan 17, 2019·Phytotherapy Research : PTR·Nana ZhangZijian Li

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Methods Mentioned

BETA
electrophoresis
PCR
fluorescence microscopy
dissection

Software Mentioned

CluxtalX1
Statistical Package for Social Sciences ( SPSS
ClustalX1
SPSS
Jalview
Expasy
SOPMA
MEGA

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