Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure

Proteins
Nídia EstrelaEduardo P Melo

Abstract

Amyloid fibers, implicated in a wide range of diseases, are formed when proteins misfold and stick together in long rope-like structures. As a natural mechanism, osmolytes can be used to modulate protein aggregation pathways with no interference with other cellular functions. The osmolyte sucrose delays fibrillation of the ribosomal protein S6 leading to softer and less shaped-defined fibrils. The molecular mechanism used by sucrose to delay S6 fibrillation was studied based on the two-state unfolding kinetics of the secondary and tertiary structures. It was concluded that the delay in S6 fibrillation results from stabilization and compaction of the slightly expanded tertiary native structure formed under fibrillation conditions. Interestingly, this compaction extends to almost all S6 tertiary structure but hardly affects its secondary structure. The part of the S6 tertiary structure that suffered more compaction by sucrose is known to be the first part to unfold, indicating that the native S6 has entered the unfolding pathway under fibrillation conditions.

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Citations

May 10, 2016·International Journal of Biological Macromolecules·Kinjal A PatelIpsita Roy
Mar 20, 2020·Journal of Biological Engineering·Farhad AbasalizadehAbolfazl Akbarzadeh
Sep 25, 2020·Communications Biology·Jingwen LiLishan Yao
Feb 26, 2021·International Journal of Biological Macromolecules·Marco A Saraiva

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