Sucrose-regulated expression of a chimeric potato tuber gene in leaves of transgenic tobacco plants

Plant Molecular Biology
H C WenzlerW D Park

Abstract

Patatin is a family of lipid acyl hydrolases that accounts for 30 to 40% of the total soluble protein in potato tubers. Class-I patatin genes encode 98 to 99% of the patatin mRNA in tubers, but are not normally expressed in other tissues. They are not totally 'tuber-specific'; however, since they can be induced to express at high levels in other tissues under conditions of sink limitation or in explants cultured on medium containing elevated levels of sucrose. To examine the evolution of the mechanisms that regulate patatin gene expression, we introduced a chimeric patatin-beta-glucuronidase (GUS) gene containing 2.5 kb of 5' flanking sequence from the Class-I potato patatin gene PS20 into tobacco plants. The construct was not expressed at significant levels in leaves of juvenile plants or plantlets cultured in vitro, but was expressed at high levels in explants cultured on medium containing 0.3 to 0.4 M sucrose. While there were differences in the expression of the chimeric gene between transgenic tobacco and potato plants, the pattern of sucrose induction was very similar. These results suggest that the mechanism that controls patatin gene expression in potato tubers evolved from a widely distributed mechanism in which gene e...Continue Reading

References

May 1, 1988·Molecular & General Genetics : MGG·D Twell, G Ooms
Jun 11, 1986·Nucleic Acids Research·M BevanG Iturriaga
Jul 11, 1986·Nucleic Acids Research·C S PikaardW D Park
Jun 5, 1985·Journal of Molecular Biology·M KreisP R Shewry
Nov 26, 1984·Nucleic Acids Research·M Bevan
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Sengupta-GopalanJ D Kemp
May 1, 1988·Plant Molecular Biology·W J StiekemaJ D Louwerse

❮ Previous
Next ❯

Citations

Feb 1, 1995·Plant Molecular Biology·S AbrahamsJ M Watson
Nov 15, 1994·Molecular & General Genetics : MGG·Z BánfalviE Barta
Oct 1, 1990·Current Opinion in Biotechnology·A Caplan, M Van Montagu
May 25, 2002·The Plant Journal : for Cell and Molecular Biology·Melina ZourelidouMichael W Bevan
May 1, 2009·Plant Physiology·Fatemeh RahmaniJohannes Hanson
Jul 1, 1991·Plant Physiology·G D ColemanL Fuchigami
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Sjef Smeekens
Jan 1, 2008·The Arabidopsis Book·Matthew RamonJen Sheen
Apr 29, 1998·Proceedings of the National Academy of Sciences of the United States of America·T J Chiou, D R Bush
Aug 1, 1990·FEBS Letters·S PratM Rocha-Sosa
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Trinh Ngoc AiChang Kil Kim
Oct 24, 2001·Plant & Cell Physiology·T FuruichiS Muto
Jan 26, 2005·Biochemical Society Transactions·A WieseS Smeekens
Sep 29, 2020·Frontiers in Plant Science·Sara Durán-SoriaJosé G Vallarino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.