Sucrose transport across the vacuolar membrane of Ananas comosus

Functional Plant Biology : FPB
Shelley R McRaeJoseph A M Holtum

Abstract

This paper originates from a presentation at the IIIrd International Congress on Crassulacean Acid Metabolism, Cape Tribulation, Queensland, Australia, August 2001. In Ananas comosus L. (Merr.) (pineapple), a widely cultivated bromeliad that exhibits crassulacean acid metabolism (CAM), much of the carbohydrate synthesized during the daytime appears to accumulate as soluble sugars in the vacuole. To investigate the mechanism of sugar transport into the vacuole, microsomal extracts were prepared from deacidifying leaves harvested during Phase III of the CAM cycle. The vesicle preparations exhibited features expected for a fraction highly enriched in vacuolar membranes (tonoplast), i.e. the ATPase activity of 16 ±2nkat mg-1 protein was inhibited 96% by 50 mm KNO3, an inhibitor of vacuolar ATPases, and was only 7% inhibited by 100μm NaN3 plus 100μm Na3VO4, inhibitors of mitochondrial and plasma membrane ATPases, respectively. Further, the microsomal ATPase activity showed a pH optimum between 7.0 and 8.0, typical of a vacuolar ATPase. When presented with Mg-ATP, vesicles established H+ gradients that could be maintained for at least 25 min. The vesicles were able to take up [14C]sucrose from an external medium. Sucrose uptake exhib...Continue Reading

Citations

Nov 3, 2015·Nature Genetics·Ray MingQingyi Yu
Jun 1, 2017·Functional Plant Biology : FPB·Paula Natália PereiraHelenice Mercier
Apr 10, 2004·Journal of Experimental Botany·Anne M Borland, Tahar Taybi
Jul 4, 2017·The Plant Journal : for Cell and Molecular Biology·Ching M WaiRay Ming
Mar 1, 2006·Functional Plant Biology : FPB·Fan S ChiuRong L Pan
Jul 1, 2005·Functional Plant Biology : FPB·Joseph A M HoltumH Ekkehard Neuhaus
Feb 20, 2007·FEBS Letters·H Ekkehard Neuhaus
Apr 28, 2009·Journal of Experimental Botany·Anne M BorlandJ Andrew C Smith
May 1, 2005·Functional Plant Biology : FPB·Moshe HubermanRaphael Goren
Apr 22, 2016·Current Opinion in Plant Biology·Anne M BorlandJohn C Cushman

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