Sugar glass fugitive ink loaded with calcium chloride for the rapid casting of alginate scaffold designs

Heliyon
Gabrielle Gauvin-RossignolAndré Bégin-Drolet

Abstract

Alginate-based hydrogels are widely used for the development of biomedical scaffolds in regenerative medicine. The use of sugar glass as a sacrificial template for fluidic channels fabrication within alginate scaffolds remains a challenge because of the premature dissolution of sugar by the water contained in the alginate as well as the relatively slow internal gelation rate of the alginate. Here, a new and simple method, based on a sugar glass fugitive ink loaded with calcium chloride to build sacrificial molds, is presented. We used a dual calcium cross-linking process by adding this highly soluble calcium source in the printed sugar, thus allowing the rapid gelation of a thin membrane of alginate around the sugar construct, followed by the addition of calcium carbonate and gluconic acid δ-lactone to complete the process. This innovative technique results in the rapid formation of "on-demand" alginate hydrogel with complex fluidic channels that could be used in biomedical applications such as highly vascularized scaffolds promoting pathways for nutrients and oxygen to the cells.

Citations

Aug 28, 2019·Materials·Clarissa TomasinaSandra Camarero-Espinosa
Feb 10, 2021·Tissue Engineering. Part B, Reviews·Marcus Alonso Cee WilliamsDeok-Ho Kim
Sep 2, 2020·Chemical Reviews·Ryan W BarrsYing Mei

❮ Previous
Next ❯

Methods Mentioned

BETA
bioprinting
scanning electron microscopy

Software Mentioned

Repetier
Marlin
- Host

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.