Sugar-Mediated Stabilization of Protein against Chemical or Thermal Denaturation

The Journal of Physical Chemistry. B
Satoshi AjitoMitsuhiro Hirai

Abstract

The protective action of sugars against the denaturation of myoglobin was clarified by X-ray and neutron scattering methods. Different types of sugars such as disaccharides (trehalose, sucrose) and monosaccharides (glucose, fructose) were used. Experimental data and theoretical simulation based on three different solvation models (preferential solvation model, nonpreferential solvation model, and preferential exclusion (hydration) model) indicated that sugar molecules were preferentially or weakly excluded from the protein surface and preserved the native protein hydration shell. This trend was more evident for disaccharides. The preferential exclusion shifted gradually to the nonpreferential solvation at higher sugar concentrations. On the protective actions of the sugars against the guanidinium-chloride-mediated denaturation, all sugars, starting from the low concentration of 5% w/v, showed the protective trend toward the protein native structure, especially for the secondary structure. The thermal structural transition temperature of myoglobin was raised by about 4-5 °C, accompanied by amyloid formation, for all hierarchical structural levels. In particular, the oligomer formation of the amyloid aggregates was more suppresse...Continue Reading

References

Dec 7, 1982·Biochemistry·T Arakawa, S N Timasheff
Jul 14, 1995·Science·Y BaiS W Englander
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
Apr 29, 1998·Annual Review of Physiology·J H CroweL M Crowe
Mar 10, 2001·Nature·M FändrichC M Dobson
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·P R Davis-SearlesG J Pielak
Jul 2, 2002·Journal of Synchrotron Radiation·Mitsuhiro HiraiKatsuaki Inoue
Mar 11, 2003·Glycobiology·Alan D ElbeinDavid Carroll
Dec 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marcus FändrichStephan Diekmann
Dec 20, 2003·Nature·Christopher M Dobson
May 25, 2005·The Journal of Chemical Physics·C BrancaJ Taylor
Aug 23, 2005·Biophysical Journal·Jörg RösgenDavid Wayne Bolen
Sep 29, 2006·The Journal of Physical Chemistry. B·Alexandra SimperlerGerhard Zifferer
Mar 1, 2007·Protein Science : a Publication of the Protein Society·Jörg RösgenDavid Wayne Bolen
Oct 13, 2009·Biochemistry·Vincent VagenendeBernhardt L Trout
Mar 29, 2011·Journal of Insect Physiology·Weronika WełniczMarcus Frohme
Mar 22, 2014·The Plant Journal : for Cell and Molecular Biology·John Edward LunnMark Stitt
Jan 30, 2015·Physical Chemistry Chemical Physics : PCCP·Philip Ball, John E Hallsworth
May 10, 2017·The European Physical Journal. E, Soft Matter·O RonsinT Baumberger

❮ Previous
Next ❯

Citations

Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hashanthi K Abeyratne-PereraPreethi L Chandran
Jul 31, 2021·Journal of Biochemical and Molecular Toxicology·Rasha Abu-KhudirAfrah F Salama
Nov 22, 2018·The Journal of Physical Chemistry. B·Mitsuhiro HiraiNobutaka Shimizu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.