Sugaring-out induced homogeneous liquid-liquid microextraction as an alternative mode for biological sample preparation: A comparative study.

Journal of Separation Science
Inas A AbdallahFotouh R Mansour

Abstract

Miniaturization of liquid-liquid extraction is a growing field of sample preparation to reduce solvent consumption, protect the environment, and preserve operators' health. In this work, four different modes of liquid-liquid microextraction have been compared including dispersive liquid-liquid microextraction, binary and ternary salting-out, and sugaring-out induced liquid-liquid microextraction. The extraction efficiency was evaluated by the enrichment factors of 14 different drugs from three pharmacological classes. Compared with the other modes, sugaring-out induced liquid-liquid microextraction was found to be the most efficient and, thus, it was applied for sample preparation of the antivirals in human plasma. Method optimization was performed using response surface methodology for the sugar type and amount (in mg), the sample pH, the equilibration time (in min), and the extractant volume (in µL). The method was then validated and found linear in the concentration range of 0.10-10 µg/mL for daclatasvir, 0.05-10 µg/mL for velpatasvir, and 0.20-10 µg/mL for ledipasvir, with correlation coefficients in the range 0.996-0.999. These results shows that sugaring-out induced liquid-liquid microextraction could be a more efficient ...Continue Reading

References

Nov 10, 2007·Journal of Chromatography. a·Yi ChenChanggui Qiu
Dec 17, 2008·Journal of Chromatography. a·Constantine StalikasTriantafyllos Albanis
Aug 13, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fotouh R Mansour, Mostafa A Khairy
Aug 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenbin ChenXijuan Tu
Dec 20, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mokhtar M MabroukFotouh R Mansour
May 11, 2021·Virusdisease·Soad S MandohFotouh R Mansour

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