PMID: 2118656Sep 1, 1990Paper

Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein

Proceedings of the National Academy of Sciences of the United States of America
G A CangelosiE W Nester

Abstract

Phenolic plant metabolites such as acetosyringone induce transcription of the virulence (vir) genes of Agrobacterium tumefaciens through the transmembrane VirA protein. We report here that certain sugars induce the vir genes synergistically with phenolic inducers by way of a distinct regulatory pathway that includes VirA and a chromosomally encoded virulence protein, ChvE. Sequence comparison showed that ChvE is a periplasmic galactose-binding protein corresponding to the GBP1 protein isolated from Agrobacterium radiobacter. Like homologous sugar-binding proteins in Escherichia coli, ChvE was required for chemotaxis toward galactose and several other sugars. These sugars strongly induced vir gene expression in wild-type cells when acetosyringone was absent or present in low concentrations. Mutations in chvE abolished vir gene induction by sugars and resulted in a limited host range for infection but did not affect vir gene induction by acetosyringone. A mutant lacking the periplasmic domain of VirA exhibited the same regulatory phenotype and limited host range as chvE mutants. These data show that the vir genes are regulated by two separate classes of plant-derived inducers by way of distinct regulatory pathways that can be sep...Continue Reading

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J W Morris, R O Morris
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S C WinansE W Nester
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J BollingerG L Hazelbauer
Jan 1, 1984·Annual Review of Biochemistry·M McNeilP Albersheim
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski

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Citations

May 1, 1992·Plant Molecular Biology·P J Hooykaas, R A Schilperoort
Nov 1, 1991·Molecular & General Genetics : MGG·C Y ChenS C Winans
Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Sep 23, 2003·Research in Microbiology·Brit WinnenMilton H Saier
Sep 13, 2001·Bioorganic & Medicinal Chemistry·J E GestwickiL L Kiessling
Jun 22, 1999·Current Opinion in Plant Biology·T Roitsch
Aug 6, 2008·Nature Reviews. Microbiology·Stacie A BrownMarvin Whiteley
Jun 20, 2002·European Journal of Biochemistry·Cristina De CastroMichelangelo Parrilli
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaozhen HuAndrew N Binns
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G J PazourA Das
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M HessA N Binns
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K LeeA N Binns
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Q PanE W Nester
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J EscuderoB Hohn
Jun 23, 2006·Infection and Immunity·Andrew J NowalkJames A Carroll
May 15, 2007·Journal of Bacteriology·Henry S GibbonsMiriam Braunstein
Jun 19, 2007·Journal of Bacteriology·Rong Gao, David G Lynn
Jun 27, 2000·Journal of Bacteriology·J ZhuS C Winans
Mar 5, 2004·Journal of Bacteriology·Avanti BaruahMichiko M Nakano

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