Sugars Require Rigid Multivalent Displays for Activation of Mouse Sperm Acrosomal Exocytosis

Biochemistry
He HuangNicole S Sampson

Abstract

As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic inducers of mouse sperm acrosomal exocytosis (AE). Each carbohydrate is proposed to have a distinct binding site on the sperm cell surface. To determine the role of the scaffold structure in the efficiency of AE induction, different polymer backbones were employed to display the different activating sugar residues. These glycopolymers were prepared by ruthenium-catalyzed ring-opening metathesis of 5-substituted norbornene or cyclooctene. The conformations of the glycopolymers were characterized by small-angle X-ray scattering. Polynorbornene displaying mannose, fucose, or GlcNAc forms flexible cylinders in aqueous solution. However, polycyclooctenes displaying any of these same sugars are much more flexible and form random coils. The flexible polycyclooctenes displaying fucose or GlcNAc were less effective inducers of AE than their norbornene counterparts. In contrast, polycyclooctene displaying mannose was the most ...Continue Reading

References

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Citations

Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiyuan Ma, X X Zhu

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Methods Mentioned

BETA
X-ray
nuclear magnetic resonance
column chromatography
light scattering
NMR
Flow
Assay
flow cytometry
SANS

Software Mentioned

SasView

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