PMID: 8608163Mar 29, 1996Paper

Suicide inactivation of porcine leukocyte 12-lipoxygenase associated with its incorporation of 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid derivative

Biochimica Et Biophysica Acta
K KishimotoT Tanabe

Abstract

Two isozymes of arachidonate 12-lipoxygenase, platelet-type and leukocyte-type, which were distinguished by their substrate specificities and primary structures, were investigated with reference to 'suicide' inactivation. Upon reaction with arachidonic acid the leukocyte-type enzyme was inactivated rapidly during the catalysis, whereas the platelet-type enzyme did not show such a rapid inactivation. The two 12-lipoxygenase isozymes were incubated with various hydroperoxy and hydroxy products from arachidonic acid. (15S)-Hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) was found to be a unique substrate of the leukocyte-type 12-lipoxygenase as follows. (1) 15-HPETE was an active substrate for porcine leukocyte 12-lipoxygenase, and converted anaerobically to a 14,15-epoxy compound (14,15-leukotriene A4). (2) A rapid inactivation of the enzyme was observed within 2 min upon aerobic and anaerobic incubations with 15-HPETE. (3) 15-HPETE was rapidly incorporated into the enzyme in a nearly equimolar amount under both aerobic and anaerobic conditions. (4) Several findings suggested a covalent binding of 15-HPETE or its derivative to the enzyme. (5) Such a rapid and stoichiometric incorporation of 15-HPETE was not observed with t...Continue Reading

References

Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T YoshimotoT Tanabe
Nov 1, 1988·Archives of Biochemistry and Biophysics·Y TakahashiS Yamamoto
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Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Tanihiro Yoshimoto, Yoshitaka Takahashi
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Hartmut KuhnRalf Jürgen Kuban
Apr 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K Shen, C A Herman
Jul 11, 2002·Free Radical Biology & Medicine·Hartmut Kühn, Astrid Borchert
Mar 27, 2007·PLoS Computational Biology·Kun YangLuhua Lai
Sep 20, 2005·Biochemical and Biophysical Research Communications·Hartmut KuhnMatthias Walther
Dec 25, 2007·Journal of Molecular Biology·Ansari M AleemEwa Skrzypczak-Jankun
May 30, 2002·Biological Chemistry·Tankred Schewe
Jan 18, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuki KawakamiYoshitaka Takahashi
Jun 13, 1998·The Journal of Biological Chemistry·M J MuellerJ Z Haeggström
Mar 1, 1997·Progress in Lipid Research·S YamamotoN Ueda
May 2, 1998·Biochimica Et Biophysica Acta·K MatsuiT Kajiwara
May 9, 2006·Progress in Lipid Research·Hartmut Kühn, Valerie B O'Donnell

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