Oct 1, 1979

Suitability of commercial control sera for the quality control of activity determination of alkaline phosphatase

Clinica Chimica Acta; International Journal of Clinical Chemistry
K JungE Egger

Abstract

The suitability of thirteen commercially available control sera for measuring alkaline phosphatase (EC 3.1.3.1; orthophosphoric acid monoester phosphohydrolase, ALP) activity in human serum was tested. Apart from differences in ALP activity observed in some reconstituted commercial sera, the behaviour of control materials towards experimental variables such as the nature and concentration of the substrate, pH and type of buffer (or PO4-acceptor) together with the composition of the isoenzymes present in human serum highlights the problems and difficulties if commercial materials are to be used as control sera. The half-saturation constants in control sera were in all cases smaller than those of ALP isoenzymes from bone and liver. The shape of substrate activity curves and the pH optimum in most of control sera differed from that of human serum. The discrepant kinetic data of control materials and human serum may mask or suggest changes relevant to commercial quality control serum but not to samples of human serum.

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References

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Citations

Mentioned in this Paper

Blood Preservation
Alkaline Phosphatase Measurement
Alkaline Phosphatase
Phosphoglycerate Dehydrogenase Activity
Blood
Esterases
Standardization
PHGDH wt Allele
Drug Stability
Serum

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