Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA.

Virology Journal
Milovan MilovanovićK Dietze

Abstract

The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of ...Continue Reading

References

Mar 10, 2001·The British Journal of Nutrition·H KorhonenH S Gill
Nov 21, 2002·Preventive Veterinary Medicine·K StåhlJ Moreno-López
May 28, 2008·Transboundary and Emerging Diseases·S BabiukD B Boyle
Mar 14, 2009·Transboundary and Emerging Diseases·S BabiukD B Boyle
Aug 17, 2010·Journal of the American Veterinary Medical Association·Dawn E MorinPeter D Constable
Jul 14, 2011·Transboundary and Emerging Diseases·E S M Tuppurainen, C A L Oura
Jun 29, 2014·Antiviral Research·Eeva S M TuppurainenPeter P C Mertens
Nov 14, 2015·Transboundary and Emerging Diseases·E S M TuppurainenL A Babiuk

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Methods Mentioned

BETA
Enzyme-linked Immunosorbent Assay
ELISA

Software Mentioned

RStudio©
Optimal Cutpoints
reshape2

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