Suitability of loci for multiple-locus variable-number of tandem-repeats analysis of Cryptosporidium parvum for inter-laboratory surveillance and outbreak investigations

Parasitology
R M ChalmersS J Hadfield

Abstract

Cryptosporidium parvum is the major cause of livestock and zoonotically-acquired human cryptosporidiosis. The ability to track sources of contamination and routes of transmission by further differentiation of isolates would assist risk assessment and outbreak investigations. Multiple-locus variable-number of tandem-repeats (VNTR) analysis provides a means for rapid characterization by fragment sizing and estimation of copy numbers, but structured, harmonized development has been lacking for Cryptosporidium spp. To investigate potential for application in C. parvum surveillance and outbreak investigations, we studied nine commonly used VNTR loci (MSA, MSD, MSF, MM5, MM18, MM19, MS9-Mallon, GP60 and TP14) for chromosome distribution, repeat unit length and heterogeneity, and flanking region proximity and conservation. To investigate performance in vitro, we compared these loci in 14 C. parvum samples by capillary electrophoresis in three laboratories. We found that many loci did not contain simple repeat units but were more complex, hindering calculations of repeat unit copy number for standardized reporting nomenclature. However, sequenced reference DNA enabled reproducible fragment sizing and inter-laboratory allele assignation...Continue Reading

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Citations

Mar 8, 2018·The Journal of Infectious Diseases·Carol A GilchristWilliam A Petri
Mar 23, 2021·Food and Waterborne Parasitology·Christine A YantaRebecca A Guy
May 28, 2021·European Journal of Protistology·Jennifer K O'LearyBrigid Lucey
May 17, 2021·Experimental Parasitology·Jennifer K O' LearyBrigid Lucey
Jul 3, 2021·Genes·Rodrigo P BaptistaJessica C Kissinger
Sep 3, 2021·Food and Waterborne Parasitology·Jennifer K O'LearyBrigid Lucey
Aug 22, 2021·The Journal of Infectious Diseases·Ines DettwilerPhilipp Olias

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