Sulfate stabilizes the folding intermediate more than the native structure of endostatin

Archives of Biochemistry and Biophysics
Yan FuYongzhang Luo

Abstract

Endostatin, a potent angiogenesis inhibitor, is an acid resistant protein with compact tertiary structure. Nuclear magnetic resonance, circular dichroism, and tryptophan emission fluorescence were used to monitor the structural changes of endostatin during acid-, heat-, and urea-induced unfolding processes. Results show that sulfate anions sensitize endostatin to acid, but specifically stabilize it against heat or urea. Moreover, the disappearance of the tertiary structure and the formation of the folding intermediate of endostatin at pH 3.0 are sulfate concentration dependent. These phenomena indicate that sulfate anions stabilize the folding intermediate more than the native structure of endostatin. In addition, heparin shows stronger effect than sodium sulfate on sensitizing endostatin against acid, and very limited stabilizing effect against urea. The loose structure of endostatin upon heparin binding may imply that the physiologically favorable structure for endostatin exerting its biological functions is not as compact as what was reported.

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y GotoA L Fink
Apr 10, 1990·Biochemistry·Y GotoA L Fink
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y H DingD C Wiley
Sep 22, 2001·Protein Science : a Publication of the Protein Society·D E Kamen, R W Woody
Apr 18, 2002·Bioorganic & Medicinal Chemistry Letters·Soko KasaiHitoshi Hori
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian J Bennion, Valerie Daggett
Mar 3, 2004·Biochemistry·Bing LiYongzhang Luo
Jul 9, 2004·Biochemical and Biophysical Research Communications·Xiaoyu WuYongzhang Luo
Nov 30, 2004·Journal of Molecular Biology·Andreas MöglichThomas Kiefhaber
Nov 5, 2005·The Journal of Biological Chemistry·Yingbo HeYongzhang Luo
Jun 1, 2006·Biochemistry·Laura SilvianAnthony Rossomando

❮ Previous
Next ❯

Citations

Feb 10, 2010·Pharmaceutical Research·Mark Cornell ManningDerrick S Katayama
Aug 2, 2016·Advanced Pharmaceutical Bulletin·Abbas MohajeriNosratollah Zarghami
May 28, 2009·IUBMB Life·Yan FuYongzhang Luo
Jul 7, 2019·Clinical and Experimental Pharmacology & Physiology·Lifang GuoYongzhang Luo
Jun 17, 2009·Biotechnology and Applied Biochemistry·Li-Ping JiangYali Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.