Sulfated glycosaminoglycans support osteoblast functions and concurrently suppress osteoclasts

Journal of Cellular Biochemistry
Juliane Salbach-HirschLorenz C Hofbauer

Abstract

In order to improve bone regeneration, development and evaluation of new adaptive biomaterials is warranted. Glycosaminoglycans (GAGs) such as hyaluronan (HA) and chondroitin sulfate (CS) are major extracellular matrix (ECM) components of bone, and display osteogenic properties that are potentially useful for biomaterial applications. Using native and synthetic sulfate-modified GAGs, we manufactured artificial collagen/GAG ECM (aECMs) coatings, and evaluated how the presence of GAGs and their degree of sulfation affects the differentiation of murine mesenchymal stem cells to osteoblasts (OB) cultivated on these aECMs. GAG sulfation regulated osteogenesis at all key steps of OB development. Adhesion, but not migration, was diminished by 50% (P < 0.001). Proliferation and metabolic activity were slightly (P < 0.05) and cell death events strongly (P < 0.001) down-regulated due to a switch from proliferative to matrix synthesis state. When exposed to sulfated GAGs, OB marker genes, such as alkaline phosphatase, osteoprotegerin (OPG), and osteocalcin increased by up to 28-fold (P < 0.05) and calcium deposition up to 4-fold (P < 0.05). Furthermore, GAG treatment of OBs suppressed their ability to support osteoclast (OC) differentiati...Continue Reading

References

Jul 1, 1985·Calcified Tissue International·C C Chen, A L Boskey
Aug 1, 1983·The Journal of Nutrition·C W Prince, J M Navia
Oct 30, 1998·Biomaterials·L L Hench
Aug 2, 2001·The Journal of Endocrinology·G P ThomasE M Gardiner
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 24, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gerald J AtkinsAndrew C W Zannettino
Jul 30, 2005·Biomaterials·Laurence Marcelle MathieuJan-Anders E Månson
Aug 12, 2008·Current Pharmaceutical Biotechnology·Stephen H Brown, Philip E Pummill
Aug 30, 2008·Regenerative Medicine·Charalampos PontikoglouPierre Charbord
Sep 5, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Martina RaunerLorenz C Hofbauer
Jan 17, 2009·Cancer Research·Francois LamoureuxFrançoise Rédini
Sep 19, 2009·The Journal of Biological Chemistry·Takashi HoshibaGuoping Chen
Jan 29, 2010·Journal of Cellular Biochemistry·Anne-Helen LutterPeter Dieter
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·A S BrydoneS Maclaine
Dec 22, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Juliane SalbachLorenz C Hofbauer
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Oct 9, 2012·Bone·Lilian I Plotkin, Teresita Bellido
Mar 20, 2013·Biomatter·Susanne BierbaumDieter Scharnweber

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Citations

Jan 15, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Michael AnsorgeTilo Pompe
Oct 10, 2015·ACS Applied Materials & Interfaces·Sandra RotherDieter Scharnweber
Sep 12, 2015·Journal of Materials Science. Materials in Medicine·Dieter ScharnweberVera Hintze
Aug 4, 2015·Biomaterials·Juliane Salbach-HirschLorenz C Hofbauer
Dec 17, 2014·Biochimica Et Biophysica Acta·Sumit BhattacharyyaJoanne K Tobacman
Jan 27, 2017·Biomacromolecules·Christian KascholkeMichael C Hacker
Feb 12, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Wolfgang MetzgerDieter Scharnweber
Sep 17, 2016·Biological Chemistry·Mareen Pagel, Annette G Beck-Sickinger
Jan 23, 2019·Stem Cells Translational Medicine·Seth AndrewsSteven Stice
Feb 26, 2019·Journal of Tissue Engineering and Regenerative Medicine·Xiaoyan RenJustine C Lee
Sep 7, 2019·Biomedical Materials·Erick K BreathwaiteJung Bok Lee
Oct 27, 2018·Frontiers in Bioengineering and Biotechnology·Robert Owen, Gwendolen C Reilly
May 3, 2019·Journal of Tissue Engineering and Regenerative Medicine·A Diez-EscuderoM-P Ginebra
Feb 23, 2017·Annual Review of Biomedical Engineering·Diana Soares da CostaIva Pashkuleva
Feb 6, 2020·International Journal of Molecular Sciences·Alexander SieberathKenny Dalgarno
Dec 31, 2019·International Journal of Biological Macromolecules·Chandra GavvaD Nandini Chilkunda
Jul 30, 2021·Biological Chemistry·Juliane Salbach-HirschLorenz C Hofbauer

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