Sulfated oligosaccharides isolated from the respiratory mucins of a secretor patient suffering from chronic bronchitis

Biochimie
Sophie DegrootePhilippe Roussel

Abstract

The most acidic carbohydrate chains released by alkaline borohydride treatment of the bulk of airway mucins secreted by a patient (blood group O, secretor) suffering from a mildly infected chronic bronchitis have been fractionated using high-performance anion-exchange chromatography (HPAEC) according to a protocol already described [Lo-Guidice et al., J. Biol. Chem. 269 (1994) 18794] and were analyzed using 1H-NMR spectroscopy and matrix-assisted laser-adsorption-time-of-flight (MALDI-TOF) spectrometry. Many fractions corresponded to mixtures of oligosaccharides. This confirmed the wide diversity of the post-translational processes involved in the biosynthesis of airway mucins, which had already been observed in bronchial diseases, such as chronic bronchitis and cystic fibrosis (CF). Seven fractions were directly purified by HPAEC, allowing their structural determination. Six of them corresponded to 3-O-sulfated oligosaccharide chains terminated by a sulfated N-acetyllactosamine, a sulfated Lewis X or a sulfated Lewis A determinant, and the last one corresponded to a 6-O-sulfated chain terminated by a sulfated H-2 determinant. Three oligosaccharides had core type 2 and the other four had core type 4: IIIc2-9: Gal(beta1-3)[HSO(3...Continue Reading

References

Jun 27, 2001·Glycoconjugate Journal·A ScharfmanJ Mazurier
Oct 2, 2001·Frontiers in Bioscience : a Journal and Virtual Library·M C CopinN Porchet
Oct 2, 2001·Frontiers in Bioscience : a Journal and Virtual Library·N MoniauxS K Batra

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Citations

Jul 8, 2008·Glycoconjugate Journal·Estelle GarenauxYann Guérardel
Jan 18, 2006·American Journal of Respiratory Cell and Molecular Biology·Olatunji W WilliamsChristopher M Evans
Dec 9, 2008·Pharmacology & Therapeutics·Christopher M Evans, Ja Seok Koo
Jun 21, 2008·Bioorganic & Medicinal Chemistry Letters·Soonsil HyunJaehoon Yu
Nov 19, 2009·The FEBS Journal·Pia H JensenNicolle H Packer
Feb 13, 2013·Carbohydrate Polymers·A T LewisP D Lewis
Oct 22, 2013·Expert Review of Respiratory Medicine·Vignesh VenkatakrishnanMorten Thaysen-Andersen
Jul 7, 2007·Physiological Reviews·Toshinori Yoshida, Rubin M Tuder

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