PMID: 2120081Oct 1, 1990Paper

Sulfated proteoglycans in Bruch's membrane of the human eye: localization and characterization using cupromeronic blue

Experimental Eye Research
T W Call, J G Hollyfield

Abstract

The distribution of sulfated proteoglycans in Bruch's membrane of the human eye was evaluated histochemically using Cupromeronic Blue in combination with specific enzyme digestions and nitrous acid treatment. Five distinct categories of filament-shaped profiles were present following staining with this dye. Type 1 (90 +/- 13 nm long and 7 +/- 1 nm in diameter) (mean +/- S.D.) and type 2 (43 +/- 7 nm long and 5 +/- 1 nm in diameter) filaments were associated with collagen fibrils in the inner and outer collagenous zones. Type 3 profiles (70 +/- 18 nm long and 8 +/- 1 nm in diameter) were present in two locations--along the cortical border of the central elastic zone and within the basal infoldings of the pigment epithelium. Type 4 (60 +/- 11 nm long and 6 +/- 1 nm in diameter) and type 5 (200 +/- 100 nm long and 100 +/- 50 nm in diameter) filaments were associated with the basal laminae of the retinal pigment epithelium and choriocapillaris. Chondroitinase AC treatment eliminated the staining of type 1 filaments. Chondroitinase ABC treatment eliminated the staining of both type 1 and type 2 filaments. Nitrous acid eliminated the staining of type 4 and type 5 filaments. Incubations with keratanase or hyaluronidase did not alter t...Continue Reading

References

Dec 1, 1985·Collagen and Related Research·J E Scott
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Jan 1, 1984·Tissue & Cell·E Essner, S R Gordon

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Citations

Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·John W CrabbJoe G Hollyfield
May 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christopher B ToomeyCatherine Bowes Rickman
Feb 16, 2013·Experimental Eye Research·Paul J Park, Deepak Shukla
Jan 28, 2003·The American Journal of Pathology·Goldis MalekChristine A Curcio
Jun 1, 1994·Microscopy Research and Technique·T H van Kuppevelt, J H Veerkamp
Nov 1, 2016·Experimental Biology and Medicine·Charles G MillerJean E Schwarzbauer
Aug 31, 2016·Animal : an International Journal of Animal Bioscience·A CarluccioA Contri
Sep 30, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Una KellyCatherine Bowes Rickman

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