Sulfatide with short fatty acid dominates in astrocytes and neurons

The FEBS Journal
Giorgis IsaacJan-Eric Månsson

Abstract

Glycosphingolipids are located in cell membranes and the brain is especially enriched. We speculated that the subcellular location of glycosphingolipids depends on their fatty acid chain length because their sugar residues are constant, whereas fatty acid chain length can vary within the same molecule. To test this hypothesis we analysed the glycosphingolipid sulfatide, which is highly abundant in myelin and has mostly long fatty acids. We used a negative ion electrospray tandem mass spectrometry precursor ion scan to analyse the molecular species of sulfatide in cultured astrocytes and a mouse model of the human disease metachromatic leukodystrophy. In these arylsulfatase A (ASA)-deficient mice sulfatide accumulates intracellularly in neurons and astrocytes. Immunocytochemistry was also performed on cultured astrocytes and analysed using confocal laser scanning microscopy. Analyses of the molecular species showed that cultured astrocytes contained sulfatide with a predominance of stearic acid (C18), which was located in large intracellular vesicles throughout the cell body and along the processes. The same was seen in ASA-deficient mice, which accumulated a higher proportion (15 mol% compared with 8 mol% in control mice) of su...Continue Reading

References

Apr 1, 1988·The Biochemical Journal·P FredmanL Svennerholm
Apr 1, 1966·Journal of Neurochemistry·W T Norton, L A Autilio
Jan 18, 1980·Biochimica Et Biophysica Acta·L Svennerholm, P Fredman
Mar 3, 1994·Biochimica Et Biophysica Acta·J P VosB M Gadella
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·B HessV Gieselmann
Jan 1, 1997·Toxicology and Applied Pharmacology·A H MerrillE Wang
Jun 19, 1998·Journal of Neuroscience Research·Z BerntsonP Fredman
Jun 26, 1998·Progress in Lipid Research·I Ishizuka
Sep 27, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Maria BlomqvistPam Fredman
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
Oct 7, 2004·Glycoconjugate Journal·Koichi HonkeNaoyuki Taniguchi
Nov 3, 2004·Journal of Neurocytology·Marie Molander-MelinPam Fredman
May 11, 2005·The Journal of Cell Biology·Delphine DelacourGuillemette Huet

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Citations

May 10, 2008·Molecular Neurobiology·Matthias Eckhardt
May 24, 2012·Journal of Lipid Research·Tadanobu Takahashi, Takashi Suzuki
Dec 4, 2009·PloS One·Karen E DrahosDaniel G S Capelluto
Apr 29, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alissa M WinzelerBen A Barres
May 22, 2007·Carbohydrate Research·José Juan Hernández-GayJesús Jiménez-Barbero
Nov 7, 2015·Neurochemical Research·Sara GrassiAlessandro Prinetti
Oct 29, 2009·Journal of Neurochemistry·Rebekka van ZylMatthias Eckhardt
Jul 12, 2016·Journal of Neuroimmunology·M Natalia MarangoniDouglas L Feinstein
May 28, 2017·Journal of Lipid Research·Maria BlomqvistMarcus Ståhlman
Jan 13, 2018·Journal of Neural Transmission·Liubov S KalinichenkoChristian P Müller
Nov 1, 2006·British Journal of Pharmacology·S Chi, Z Qi

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