Sulfide-Induced Dissimilatory Nitrate Reduction to Ammonium Supports Anaerobic Ammonium Oxidation (Anammox) in an Open-Water Unit Process Wetland

Applied and Environmental Microbiology
Zackary L JonesJonathan O Sharp

Abstract

Open-water unit process wetlands host a benthic diatomaceous and bacterial assemblage capable of nitrate removal from treated municipal wastewater with unexpected contributions from anammox processes. In exploring mechanistic drivers of anammox, 16S rRNA gene sequencing profiles of the biomat revealed significant microbial community shifts along the flow path and with depth. Notably, there was an increasing abundance of sulfate reducers (Desulfococcus and other Deltaproteobacteria) and anammox microorganisms (Brocadiaceae) with depth. Pore water profiles demonstrated that nitrate and sulfate concentrations exhibited a commensurate decrease with biomat depth accompanied by the accumulation of ammonium. Quantitative PCR targeting the anammox hydrazine synthase gene, hzsA, revealed a 3-fold increase in abundance with biomat depth as well as a 2-fold increase in the sulfate reductase gene, dsrA These microbial and geochemical trends were most pronounced in proximity to the influent region of the wetland where the biomat was thickest and influent nitrate concentrations were highest. While direct genetic queries for dissimilatory nitrate reduction to ammonium (DNRA) microorganisms proved unsuccessful, an increasing depth-dependent do...Continue Reading

References

Aug 3, 2002·Water Research·A Olav SliekersM S M Jetten
Apr 11, 2003·Nature·Allan H Devol
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·Shuangya ChenXiuzhu Dong
Jul 8, 2005·Applied and Environmental Microbiology·Noah FiererRobert B Jackson
Oct 6, 2005·Applied and Environmental Microbiology·Rikke Louise MeyerDiane Elizabeth Allen
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Sep 9, 2006·Environmental Microbiology·Carsten J SchubertMarcel M M Kuypers
Jan 12, 2007·Nature·Douglas G Capone, Angela N Knapp
Feb 27, 2007·Trends in Microbiology·Kerry S Smith, Cheryl Ingram-Smith
Mar 21, 2007·Applied and Environmental Microbiology·Marlene Mark JensenTage Dalsgaard
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Phyllis LamMarcel M M Kuypers
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Feb 16, 2010·International Journal of Systematic and Evolutionary Microbiology·Suzanna L BräuerStephen H Zinder
Mar 3, 2010·Applied Microbiology and Biotechnology·Guibing ZhuChengqing Yin
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Nov 13, 2010·Science·Don E CanfieldOsvaldo Ulloa
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anja KampPeter Stief
Aug 16, 2011·Applied and Environmental Microbiology·Francisca A LueskenBoran Kartal
Oct 11, 2011·Environmental Science & Technology·Guibing ZhuChengqing Yin
Dec 6, 2011·Applied and Environmental Microbiology·Harry R HarhangiHuub J M Op den Camp
Mar 10, 2012·Frontiers in Microbiology·Michael PesterAlexander Loy
Mar 9, 2013·Environmental Science & Technology·Justin T Jasper, David L Sedlak
Mar 14, 2013·FEMS Microbiology Ecology·Aura Ontiveros-ValenciaRosa Krajmalnik-Brown
Apr 2, 2013·Biotechnology Letters·Ziye HuBoran Kartal
Aug 29, 2013·Environmental Engineering Science·Justin T JasperKara L Nelson
Jan 28, 2014·Applied and Environmental Microbiology·Allana WelshRobert A Sanford
Apr 11, 2014·FEMS Microbiology Ecology·Nicole J BaleJaap S Sinninghe Damsté
Apr 17, 2014·Environmental Science & Technology·Justin T JasperDavid L Sedlak

❮ Previous
Next ❯

Citations

Dec 7, 2018·The ISME Journal·Guibing ZhuLorenz Schwark
Sep 14, 2019·Applied Microbiology and Biotechnology·Pedro Soares-CastroPedro M Santos
Dec 10, 2020·Environmental Microbiology·Jeseth Delgado VelaGregory J Dick
Oct 17, 2018·Water Research·Oksana CobanBrad M Bebout
Jun 17, 2020·The Science of the Total Environment·C B PandeyJ S Singh
May 12, 2018·Environmental Science & Technology·Shanyun WangGuibing Zhu
Oct 27, 2021·Environmental Science and Pollution Research International·Xiaoling LiChunshuang Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.