Sulfide Protects [FeFe] Hydrogenases From O2

Journal of the American Chemical Society
Patricia Rodríguez-MaciáJames A Birrell

Abstract

[FeFe] hydrogenases catalyze proton reduction and hydrogen oxidation with high rates and efficiency under physiological conditions, but are highly oxygen sensitive. The [FeFe] hydrogenase from Desulfovibrio desulfuricans ( DdHydAB) can be purified under air in an oxygen stable inactive state Hoxair. The formation of the Hoxair state in vitro allows the handling of hydrogenases in air, making their implementation in biotechnological applications more feasible. Here, we report a simple and robust protocol for the formation of the Hoxair state in DdHydAB and the [FeFe] hydrogenase from Chlamydomonas reinhardtii, which is based on high potential inactivation in the presence of sulfide.

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Citations

Aug 14, 2019·Biotechnology and Bioengineering·Lu Yuan, Jamin Koo
Aug 14, 2020·Chemical Communications : Chem Comm·Alaa A OughliNicolas Plumeré
Oct 14, 2018·International Journal of Molecular Sciences·Marco BortolusDonatella Carbonera
Jan 21, 2021·Chemical Communications : Chem Comm·Christina FelbekChristophe Léger
Dec 10, 2019·Journal of the American Chemical Society·Jacob H ArtzJohn W Peters
Feb 4, 2021·Nature Communications·Martin WinklerThomas Happe
Apr 13, 2021·Nature Catalysis·Steffen HardtNicolas Plumeré
Dec 11, 2019·Journal of the American Chemical Society·James A BirrellSerena DeBeer
Oct 15, 2019·Journal of the American Chemical Society·Julian EsselbornThomas Happe

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