Sulfide Toxicity and Its Modulation by Nitric Oxide in Bovine Pulmonary Artery Endothelial Cells

Chemical Research in Toxicology
Kristin L FrawleyJim Peterson

Abstract

Bovine pulmonary artery endothelial cells (BPAEC) respond in a dose-dependent manner to millimolar (0-10) levels of sodium sulfide (NaHS). No measurable increase in caspase-3 activity and no change in the extent of autophagy (or mitophagy) were observed in BPAEC. However, lactate dehydrogenase levels increased in the BPAEC exposed NaHS, which indicated necrotic cell death. In the case of galactose-conditioned BPAEC, the toxicity of NaHS was increased by 30% compared to that observed in BPAEC maintained in the regular glucose-containing culture medium, which indicated a link between mitochondrial oxidative phosphorylation and the mechanism of toxicant action. This is consistent with the widely held view that cytochrome c oxidase (complex IV of the mitochondrial electron-transport system) is the principal molecular target involved in the acute toxicity of "sulfide" (H2S/HS-). In support of this view, elevated NO (which can reverse cytochrome c oxidase inhibition) ameliorated the toxicity of NaHS and, conversely, suppression of endogenous NO production exacerbated the observed toxicity. Respirometric measurements showed the BPAEC to possess a robust sulfide oxidizing system, which was able to out-compete cytochrome c oxidase for a...Continue Reading

References

Jan 1, 1992·Annual Review of Pharmacology and Toxicology·R J ReiffensteinS H Roth
Mar 1, 1995·The American Journal of Emergency Medicine·J W SnyderR A Middleberg
Mar 7, 1996·The New England Journal of Medicine·U ScherrerP Bärtsch
Jul 1, 1996·Chemical Research in Toxicology·J S Beckman
Oct 1, 1996·Occupational Medicine·T L Guidotti
Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·F HinderJ Meyer
Jan 23, 1999·American Journal of Industrial Medicine·T H Milby, R C Baselt
Sep 9, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·H Witschi
Sep 9, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·A F Almeida, T L Guidotti
Feb 24, 2001·Wilderness & Environmental Medicine·A OmuraT Jennings
Dec 26, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·David C DormanMelanie F Struve
Dec 31, 2005·The New England Journal of Medicine·Lorraine B Ware, Michael A Matthay
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Apr 16, 2008·The American Journal of Emergency Medicine·Chaethana Yalamanchili, Michael D Smith
Apr 22, 2008·Methods in Molecular Biology·Isei TanidaEiki Kominami
Oct 7, 2008·The Journal of Biological Chemistry·Martin FeelischHouman Ashrafian
Oct 30, 2008·Chemical Research in Toxicology·Linda L PearceJim Peterson
Mar 20, 2009·Forensic Science International : Synergy·Seong Hwan ParkJuck-Joon Hwang
Jun 23, 2009·Respiratory Physiology & Neurobiology·Philippe HaouziBernard Bihain
Aug 14, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jirí SedyEva Syková
Sep 4, 2009·Archives of Pharmacal Research·Jon O Lundberg, Eddie Weitzberg
Oct 30, 2010·Antioxidants & Redox Signaling·Frédéric Bouillaud, François Blachier
Dec 17, 2010·Forensic Science, Medicine, and Pathology·Christopher Milroy, Jacqueline Parai
Jan 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hui-Hua LiLi-Ming Zhang
Jun 28, 2011·Biochimica Et Biophysica Acta·Tatjana M Hildebrandt
Jul 17, 2012·Cytotechnology·Alexander P Demchenko
Jun 5, 2014·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Philippe HaouziCandice M Klingerman
Jun 15, 2014·Nitric Oxide : Biology and Chemistry·Nada HelmyFrédéric Bouillaud
Jul 13, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Philippe HaouziCandice M Klingerman
Mar 3, 2015·Methods in Enzymology·Abbas Abou-HamdanFrédéric Bouillaud
May 8, 2015·Chemical Research in Toxicology·Andrea A CronicanJim Peterson
May 13, 2015·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Takashi Sonobe, Philippe Haouzi
Oct 12, 2017·Nitric Oxide : Biology and Chemistry·Samantha L Malone RubrightJim Peterson

❮ Previous
Next ❯

Citations

Mar 20, 2021·Antioxidants & Redox Signaling·Dario PacittiMatthew Whiteman
Jan 11, 2020·Chemical Research in Toxicology·Kristin L FrawleyJim Peterson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms