Sulfolipid-1 biosynthesis restricts Mycobacterium tuberculosis growth in human macrophages.

ACS Chemical Biology
Sarah A GilmoreCarolyn R Bertozzi

Abstract

Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is a highly evolved human pathogen characterized by its formidable cell wall. Many unique lipids and glycolipids from the Mtb cell wall are thought to be virulence factors that mediate host-pathogen interactions. An intriguing example is Sulfolipid-1 (SL-1), a sulfated glycolipid that has been implicated in Mtb pathogenesis, although no direct role for SL-1 in virulence has been established. Previously, we described the biochemical activity of the sulfotransferase Stf0 that initiates SL-1 biosynthesis. Here we show that a stf0-deletion mutant exhibits augmented survival in human but not murine macrophages, suggesting that SL-1 negatively regulates the intracellular growth of Mtb in a species-specific manner. Furthermore, we demonstrate that SL-1 plays a role in mediating the susceptibility of Mtb to a human cationic antimicrobial peptide in vitro, despite being dispensable for maintaining overall cell envelope integrity. Thus, we hypothesize that the species-specific phenotype of the stf0 mutant is reflective of differences in antimycobacterial effector mechanisms of macrophages.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M B GorenJ A Armstrong
Jun 6, 1991·Nature·C K StoverG F Hatfull
Nov 1, 1988·Infection and Immunity·L ZhangB R Andersen
Oct 1, 1974·Infection and Immunity·M Kato, M B Goren
Jan 1, 1997·Annual Review of Immunology·M BaggioliniB Moser
May 29, 2002·Chemistry & Biology·David E MinnikinGurdyal S Besra
Jan 8, 2003·The Journal of Experimental Medicine·Teunis B H GeijtenbeekYvette Van Kooyk
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott E ConverseJeffery S Cox
Mar 6, 2004·The Journal of Biological Chemistry·Pilar DomenechClifton E Barry
Jul 20, 2004·Nature Structural & Molecular Biology·Joseph D MougousCarolyn R Bertozzi
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joseph D MougousCarolyn R Bertozzi
Dec 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·G MiddlebrookW B Schaefer
Jun 17, 2006·Nature Reviews. Microbiology·Andreas Peschel, Hans-Georg Sahl
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Madhulika JainJeffery S Cox
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Pawan KumarCarolyn R Bertozzi
Jul 12, 2007·Seminars in Immunopathology·Katherine Radek, Richard Gallo
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip T LiuRobert L Modlin
Jan 5, 2008·The Journal of Organic Chemistry·Clifton D Leigh, Carolyn R Bertozzi
Aug 15, 2008·Trends in Microbiology·Annemieke M C ten BokumNeil G Stoker
Mar 12, 2009·The Journal of Biological Chemistry·Stavroula K HatziosCarolyn R Bertozzi
Sep 5, 2009·Advances in Applied Microbiology·Devinder KaurMary Jackson

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Citations

Sep 13, 2015·Protein Science : a Publication of the Protein Society·Tsung-Han ChouEdward W Yu
Aug 21, 2014·Chembiochem : a European Journal of Chemical Biology·Bailey L UrbanekBenjamin M Swarts
Jun 11, 2014·Critical Reviews in Biochemistry and Molecular Biology·Shiva Kumar AngalaMary Jackson
Mar 15, 2014·Critical Reviews in Biochemistry and Molecular Biology·Luis E N Quadri
Jan 21, 2014·Chemical Communications : Chem Comm·Danny Geerdink, Adriaan J Minnaard
Nov 8, 2016·New Microbes and New Infections·M N Gonzalez-PerezA Tauch
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Landry BlancJérôme Nigou
May 18, 2018·Future Microbiology·Parul SinghSangita Mukhopadhyay
Dec 12, 2017·Pure and Applied Chemistry. Chimie Pure Et Appliquée·Mara K O'NeillBenjamin M Swarts
Aug 1, 2015·The Journal of Biological Chemistry·Melissa Richard-GreenblattYossef Av-Gay
Dec 1, 2019·The Journal of Biological Chemistry·Yanyan Li, Sylvie Rebuffat
Aug 16, 2014·The Journal of Biological Chemistry·Juan Manuel BelardinelliMary Jackson
Sep 8, 2019·Scientific Reports·Manjari MishraShobhna Kapoor
May 27, 2016·The Journal of Biological Chemistry·Nivedita LahiriD Branch Moody
Jun 24, 2014·Organic & Biomolecular Chemistry·Chia-Hui Wu, Cheng-Chung Wang
Apr 5, 2018·Frontiers in Microbiology·Victoria L CampodónicoPetros C Karakousis
May 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chih-Chia SuEdward W Yu
Feb 2, 2021·The Journal of Biological Chemistry·Yanyan Li, Sylvie Rebuffat
Dec 15, 2020·Journal of Medicinal Chemistry·Gautam KumarShobhna Kapoor
Jun 25, 2015·Microbiology Spectrum·Mamadou DafféMary Jackson
Mar 11, 2017·Microbiology Spectrum·Melissa Richard-Greenblatt, Yossef Av-Gay
Jun 10, 2017·Microbiology Spectrum·Tracy L Keiser, Georgiana E Purdy
Jul 1, 2021·Chemical Reviews·Mira HolzheimerAdriaan J Minnaard

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Methods Mentioned

BETA
ELISA
sulfotransferase

Software Mentioned

XMASS

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