Sulfonamide inhibition studies of the γ-carbonic anhydrase from the Antarctic cyanobacterium Nostoc commune

Bioorganic & Medicinal Chemistry
Daniela VulloClaudiu T Supuran

Abstract

A carbonic anhydrase (CA, EC 4.2.1.1) belonging to the γ-class has been cloned, purified and characterized from the Antarctic cyanobacterium Nostoc commune. The enzyme showed a good catalytic activity for the physiologic reaction (hydration of carbon dioxide to bicarbonate and a proton) with the following kinetic parameters, kcat of 9.5×10(5)s(-1) and kcat/KM of 8.3×10(7)M(-1)s(-1), being the γ-CA with the highest catalytic activity described so far. A range of aromatic/heterocyclic sulfonamides and one sulfamate were investigated as inhibitors of the new enzyme, denominated here NcoCA. The best NcoCA inhibitors were some sulfonylated sulfanilamide derivatives possessing elongated molecules, aminobenzolamide, acetazolamide, benzolamide, dorzolamide, brinzolamide and topiramate, which showed inhibition constants in the range of 40.3-92.3nM. As 1,5-bisphosphate carboxylase/oxygenase (RubisCO) and γ-CAs are closely associated in carboxysomes of cyanobacteria for enhancing the affinity of RubisCO for CO2 and the efficiency of photosynthesis, investigation of this new enzyme and its affinity for modulators of its activity may bring new insights in these crucial processes.

References

Jan 3, 2008·Nature Reviews. Drug Discovery·Claudiu T Supuran
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Mar 1, 2009·Marine Genomics·Viviana De LucaClemente Capasso
Aug 3, 2011·Future Medicinal Chemistry·Claudiu T Supuran
Apr 4, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Nov 15, 2012·Expert Opinion on Therapeutic Patents·Andrea ScozzafavaClaudiu T Supuran
Jan 17, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Feb 6, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Feb 16, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Fortunato MigliardiniClemente Capasso
Mar 7, 2013·Expert Opinion on Therapeutic Patents·Claudiu T Supuran
Mar 16, 2013·Expert Opinion on Therapeutic Patents·Fabrizio Carta, Claudiu T Supuran
Apr 24, 2013·Expert Opinion on Therapeutic Patents·Andrea ScozzafavaFabrizio Carta
May 1, 2013·Expert Opinion on Drug Discovery·Giuseppina De SimoneClaudiu T Supuran
May 1, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Clemente Capasso, Claudiu T Supuran
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Aug 7, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Jan 25, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Mar 29, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Ahmed M Alafeefy
Jul 10, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Viviana De LucaClemente Capasso
Jul 23, 2014·ACS Medicinal Chemistry Letters·Mariangela CerusoClaudiu T Supuran
Aug 1, 2014·Future Medicinal Chemistry·Fabrizio CartaAndrea Scozzafava

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Citations

Dec 2, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Mar 18, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Süleyman YılmazClaudiu T Supuran
Jul 3, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Parham TaslimiClaudiu T Supuran
Jul 16, 2015·Bioorganic & Medicinal Chemistry Letters·Daniela VulloClaudiu T Supuran
Jan 20, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Parham TaslimiSabir Mamedov
Jul 18, 2017·Pathogens·Claudiu T Supuran, Clemente Capasso
Nov 2, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Erol LicsandruClaudiu T Supuran
Nov 16, 2017·Metabolites·Claudiu T Supuran, Clemente Capasso
Jul 6, 2018·Expert Opinion on Therapeutic Patents·Claudiu T Supuran, Clemente Capasso
Feb 3, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Claudiu T Supuran
Aug 10, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Aug 19, 2020·Expert Opinion on Therapeutic Patents·Claudiu T Supuran, Clemente Capasso
Jan 13, 2021·International Journal of Molecular Sciences·Viviana De LucaClemente Capasso
Apr 6, 2021·Frontiers in Microbiology·Cristina CampestreClemente Capasso
May 1, 2021·International Journal of Molecular Sciences·Claudiu T Supuran, Clemente Capasso

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