Sulforaphane effects on oxidative stress parameters in culture of adult cardiomyocytes.

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Giana Blume CorssacAdriane Belló-Klein

Abstract

The aim of this study was to analyse the effect of sulforaphane (SFN) in cultures of adult cardiomyocytes, evaluating oxidative stress at different times. Cells were isolated, cultured, and divided into 4 groups: Control, SFN (5μM), H2O2 (5μM), and SFN+H2O2 (5μM both), and subdivided into groups undergoing 1 or 24 h of SFN incubation. After 1 h of incubation, reactive oxygen species production was 40% lower in the SFN group than the Control, and lipid peroxidation was 63% higher in the H2O2 group than the Control, and it was reduced in both of the SFN groups. The SOD activity was 59% higher in groups incubated for 24 h than in those incubated for 1 h. Protein expression of SOD-1 and SOD-2 was higher in the 24-h groups compared to the 1-h groups (55% and 24%, respectively). The Nrf2 protein expression in the 1-h groups was 17% higher than in the 24-h groups, and the SFN + H2O2 group had 40% more Nrf2 than the Control in the 1-h groups. Unlike Nrf2, the PGC-1α expression was 69% higher in the 24-h groups in relation to the 1-h groups. Regarding the 24-h groups, the SFN and SFN+H2O2 groups were higher than the Control (32% and 33%, respectively), and the SFN+H2O2 group was increased (21%) compared to H2O2. SFN had a protective act...Continue Reading

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Citations

Oct 8, 2019·Antioxidants & Redox Signaling·Alma MartelliVincenzo Calderone
Oct 21, 2020·Cellular and Molecular Neurobiology·Larissa Daniele BoberminAndré Quincozes-Santos
Nov 19, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Parham Jabbarzadeh KaboliZhangang Xiao
Jun 7, 2021·Journal of Biotechnology·Martina ZimmermannAline Zimmer

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