Abstract
The purpose of this study was to determine the effect of sulforaphane (SFN) on hepatic ischemia reperfusion injury (HIRI) and to explore the underlying mechanisms. The rat model of HIRI was established. Thirty-two rats were randomly divided into sham (A), SFN (B), HIRI (C), and SFN + HIRI (D) groups. Animals in the HIRI and Sham groups were treated with equal volumes of the vehicle of SPF. Liver functions were evaluated by serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver samples were collected for histological examination and determination of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels. Mitochondrial Na(+)-K(+)-ATPase and Ca(2+)-ATPase were measured by colorimetry. Expression levels of NQO1, Nrf2, and HO-1 in liver tissue were detected by western blot analysis. Additionally, oncosis and apoptosis were detected by Annexin V-FITC/PI immunofluorescent flow cytometry analysis. The HIRI group showed a significant increase in serum levels of liver enzymes (ALT, AST) associated with histopathological damage in the liver. Pre-treatment with SFN could reduce the levels of MDA and MPO in liver tissue and improve the activities of SOD, GSH...Continue Reading
References
Sep 1, 1995·Annals of Neurology·M F Beal
Jun 1, 1993·Transplantation·S SuzukiD Cejalvo
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J W Fahey, P Talalay
Jun 27, 2001·American Journal of Surgery·F Serracino-InglottR T Mathie
Dec 21, 2002·Current Cardiology Reports·Jeffrey Leppo
Sep 18, 2003·ILAR Journal·Russell J Borski, Ronald G Hodson
Nov 3, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Toshihiro YorozuyaTatsuru Arai
Aug 27, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Georgios K GlantzounisAlexander M Seifalian
Aug 22, 2006·Current Pharmaceutical Design·D GalarisP Korantzopoulos
Apr 15, 2008·Biochemical Pharmacology·Ha-Yong YoonByung-Hyun Park
Feb 4, 2009·Antioxidants & Redox Signaling·David F Stowe, Amadou K S Camara
Jun 10, 2009·The American Journal of Chinese Medicine·Shiqing LinXuanhong Zhang
Dec 23, 2009·Toxicology and Applied Pharmacology·Tomokazu OhnumaAkira Hiratsuka
Mar 13, 2010·Biochemical Pharmacology·Dong YanShao-yu Chen
Apr 27, 2010·Brain Research·Zhang PingXuejun Sun
Jun 24, 2010·World Journal of Gastroenterology : WJG·Hai-Dong ZhaoXiao-Feng Tian
Oct 23, 2010·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Katrin HoffmannPeter Schemmer
Dec 7, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Carlos Enrique Guerrero-BeltránYolanda Irasema Chirino
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Hartmut Jaeschke
Jan 10, 2012·Free Radical Biology & Medicine·Bing WangWangsen Cao
Feb 14, 2012·Free Radical Biology & Medicine·Rowan F van GolenMichal Heger
Mar 31, 2012·Transplantation Reviews·Hartmut Jaeschke, Benjamin L Woolbright
May 3, 2012·Medicinal Research Reviews·Sadagopan MageshLongqin Hu
May 29, 2012·Seminars in Cell & Developmental Biology·Shyamal C BirChristopher G Kevil
Dec 12, 2012·PloS One·Rongchuan YueZhengping Yu
Dec 12, 2012·Nature Reviews. Gastroenterology & Hepatology·Yuan ZhaiJerzy W Kupiec-Weglinski
Sep 21, 2013·Annals of Transplantation : Quarterly of the Polish Transplantation Society·Albertas CekauskasPeter Schemmer
Oct 16, 2013·Annals of Transplantation : Quarterly of the Polish Transplantation Society·Zhanqing LiPeter Schemmer
Citations
Sep 27, 2015·Free Radical Biology & Medicine·Miles SarillCarolyn J Baglole
Jul 2, 2016·The Journal of Surgical Research·Sezgin ZerenSukru Aydin Duzgun
Apr 18, 2015·Toxicology and Applied Pharmacology·Marisanta Del RegnoStefania Marzocco
Mar 19, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Arumugam Suyavaran, Chinnasamy Thirunavukkarasu
Jan 24, 2017·Canadian Journal of Physiology and Pharmacology·Entsar A NazmyEman Said
Mar 17, 2017·Journal of Cellular and Molecular Medicine·Chenxia Hu, Lanjuan Li
May 18, 2017·British Journal of Pharmacology·Yimeng ZhangJinyong Peng
Jul 12, 2016·Nutrients·Xufeng TaoJinyong Peng
Nov 12, 2016·Human & Experimental Toxicology·W LiuYu Deng
Jul 15, 2017·Journal of Cellular Biochemistry·Xinli HuangSen Lu
Nov 5, 2015·International Journal of Molecular Medicine·Tianjie QiHaitao Li
Jun 4, 2020·Bioscience, Biotechnology, and Biochemistry·Sho HottaKenji Ichihara
Jan 5, 2017·Inflammopharmacology·Ahmed E KhodirHatem A Salem
Nov 27, 2019·Antioxidants·Ruheea Taskin RuheeKatsuhiko Suzuki
Jun 14, 2019·Plant Molecular Biology·Diana L ZuluagaMark G M Aarts
Jul 28, 2020·International Journal of Molecular Sciences·Zhiquan ChenFelix Becker
Jul 6, 2017·Archives of Toxicology·Hee Young KwonSung-Hoon Kim
Jun 23, 2020·BioMed Research International·Weidong GaoCijun Peng
May 29, 2018·Experimental and Therapeutic Medicine·Zongjian SunShiqiang Shan
Oct 18, 2020·BMJ Open·Fangkun LiuZhixiong Liu
Feb 26, 2021·European Journal of Pharmacology·Hongyan MaEnyou Li
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Sukumaran SanjayZachariah Bobby
Jun 3, 2021·Antioxidants·Bercis Imge UcarLuciano Saso
Jun 27, 2021·The Journal of Surgical Research·Zhihui GuanFeifei Shao
Jun 6, 2019·Aging and Disease·Wenjun TuHong Sha
Aug 26, 2021·Autoimmunity·Suhua ZhangWuQiang Ma
Oct 16, 2021·Experimental and Therapeutic Medicine·Yi ZhouSongqing He