Sulforaphane Reduces Prostate Cancer Cell Growth and Proliferation In Vitro by Modulating the Cdk-Cyclin Axis and Expression of the CD44 Variants 4, 5, and 7.

International Journal of Molecular Sciences
Jochen RutzRoman A Blaheta

Abstract

Prostate cancer patients whose tumors develop resistance to conventional treatment often turn to natural, plant-derived products, one of which is sulforaphane (SFN). This study was designed to determine whether anti-tumor properties of SFN, identified in other tumor entities, are also evident in cultivated DU145 and PC3 prostate cancer cells. The cells were incubated with SFN (1-20 µM) and tumor cell growth and proliferative activity were evaluated. Having found a considerable anti-growth, anti-proliferative, and anti-clonogenic influence of SFN on both prostate cancer cell lines, further investigation into possible mechanisms of action were performed by evaluating the cell cycle phases and cell-cycle-regulating proteins. SFN induced a cell cycle arrest at the S- and G2/M-phase in both DU145 and PC3 cells. Elevation of histone H3 and H4 acetylation was also evident in both cell lines following SFN exposure. However, alterations occurring in the Cdk-cyclin axis, modification of the p19 and p27 proteins and changes in CD44v4, v5, and v7 expression because of SFN exposure differed in the two cell lines. SFN, therefore, does exert anti-tumor properties on these two prostate cancer cell lines by histone acetylation and altering the ...Continue Reading

References

Apr 1, 2011·Clinical & Experimental Metastasis·Steffen WedelRoman A Blaheta
Jul 2, 2011·Molecular Medicine Reports·Mi-Ran KimJung Ryul Kim
Dec 4, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hyun Soo ParkYung Hyun Choi
May 20, 2014·Asia-Pacific Journal of Clinical Oncology·Grace V EdwardsThomas P Shakespeare
Sep 16, 2016·The American Journal of Chinese Medicine·Kuo-Ching LiuJing-Gung Chung
Mar 21, 2017·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Eva JuengelRoman A Blaheta
Oct 27, 2017·Cellular and Molecular Life Sciences : CMLS·Qingdi Quentin LiPiyush K Agarwal
Dec 22, 2017·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazumasa KomuraPhilip W Kantoff
Sep 27, 2018·BMC Complementary and Alternative Medicine·Loisa DrozdoffDaniela Paepke
Jan 30, 2019·Genes & Development·Honghong ZhangChonghui Cheng
Mar 27, 2019·Cancer Treatment Reviews·Fred SaadIra A Jacobs
May 10, 2019·World Journal of Oncology·Prashanth Rawla
May 16, 2019·The Journal of Urology·Kyle B ZunigaJune M Chan
May 8, 2020·Complementary Therapies in Clinical Practice·İsmail ToygarAbdullah Aslan
May 14, 2020·Breast Care·Friederike HammersenAnnika Waldmann
Aug 8, 2020·International Journal of Molecular Sciences·Saira JustinRoman A Blaheta

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Methods Mentioned

BETA
acetylation
ELISA
flow cytometry
enzyme-linked immunosorbent assay

Software Mentioned

Quest
GIMP
CellQuest
Cell
ModFit

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