Abstract
Cholangiocarcinoma (CCC) is an aggressive malignancy with poor therapeutic options and pronounced chemotherapy resistance. The bioactive broccoli substance, sulforaphane (SFN), is a promising new therapeutic option since it has been found to induce therapeutic effects in both experimental and epidemiological studies in various tumor entities. Thus, the present study was designed to assess the effect of SFN on cisplatin sensitivity in CCC. Human HuCCT-1 and TFK-1 cells, representing intrahepatic and extrahepatic CCC, respectively, were treated with cisplatin and SFN. Viability, the platinated DNA content, and apoptosis were assessed using both MTT assay and flow cytometry, while western blotting was used to analyze the expression of proteins involved in apoptosis and DNA damage. Whereas cisplatin was largely ineffective, SFN only therapy significantly decreased the viability of both CCC cell lines. The combination of SFN with cisplatin increased cisplatin cytotoxicity, which was particularly pronounced relatively early at 36 h after treatment. Apoptosis, which was reflected by the cleavage of caspase-3 and PARP, was significantly enhanced. Notably, only cisplatin was found to induce the expression of proteins involved in the DNA...Continue Reading
References
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangG H Posner
Jun 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M MiyagiwaH Sasaki
Sep 1, 1995·The Tohoku Journal of Experimental Medicine·S SaijyoS Matsuno
Oct 1, 1996·Annals of Surgery·A NakeebJ L Cameron
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Feb 24, 2001·Phytochemistry·J W FaheyP Talalay
Jan 10, 2002·Genome Research·Tobias DoerksPeer Bork
Oct 25, 2003·Oncogene·Zahid H Siddik
Nov 10, 2004·The Journal of Biological Chemistry·Christopher E HeltMichael A O'Reilly
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Dec 22, 2006·Carcinogenesis·Cheung-Yun JinGi-Young Kim
Feb 28, 2007·British Journal of Cancer·F Eckel, R M Schmid
Mar 6, 2007·Critical Reviews in Oncology/hematology·David J Stewart
Mar 31, 2007·Cellular and Molecular Life Sciences : CMLS·N JugeM Traka
Apr 4, 2007·Annals of the New York Academy of Sciences·Carmela FimognariPatrizia Hrelia
Apr 26, 2007·Annals of Surgery·Michelle L DeOliveiraRichard D Schulick
Feb 5, 2010·Leukemia·L-J MahT C Karagiannis
Jun 10, 2010·Cancer Research·Vanessa RauschIngrid Herr
Sep 3, 2010·Journal of Nucleic Acids·Monika PodhoreckaPrzemyslaw Bozko
Oct 14, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Georgios KallifatidisIngrid Herr
Oct 20, 2010·Cancer Epidemiology·Chhavi SharmaArif Hussain
Mar 12, 2013·Journal of Oncology·Huaping ChenTrygve O Tollefsbol
Feb 13, 2014·World Journal of Surgery·Qian D ZhuZheng P Yu
Aug 28, 2014·Toxicology Letters·Luba HunakovaJan Sedlak
Nov 22, 2014·Deutsches Ärzteblatt International·Arndt VogelUlf Neumann
Apr 14, 2015·Molecular Cell·Xinjian LiuChuan-Yuan Li
May 2, 2015·PloS One·Zai-Fa HongXiao-Min Wang
Jul 16, 2015·Clinical Sarcoma Research·G M O'KaneD N Carney
Aug 1, 2015·BMC Cancer·Alexander SteinHenning Wege
Nov 12, 2015·Thoracic Cancer·Yuankai ShiXiaohong Han
Jan 1, 2014·ISRN Hepatology·Rocio I R Macias