Sulforaphane suppresses LPS-induced inflammation in primary rat microglia.

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Lars-Ove BrandenburgChristoph J Wruck

Abstract

The aim of this study was to investigate the signal transduction pathways involved in sulforaphane (SF) mediated inhibition of the inflammatory response to lipopolysaccharide (LPS). Additionally, we investigated the effects of SF and LPS on the activity of Nrf2. Primary rat microglia and the murine microglia cell line BV2 were used. Cells were treated with LPS with or without SF. Cell viability was measured via WST-assay. Real-time RT-PCR was performed to analyze cytokine mRNA levels. The nitric oxide (NO) release was measured in LPS-stimulated microglia. The induction of various signal transduction pathways and Nrf2 was determined by Western blotting. NF-kappaB and AP-1 activation was measured by dual luciferase assay. We showed that SF attenuates the LPS-induced increase of IL-1beta, IL-6, and TNF-alpha expression in microglia. In addition, SF significantly decreases the NO in a concentration-dependent manner. SF inhibits LPS-stimulated ERK1/2 and JNK phosphorylation and thereby inhibits the LPS-induced activation of NF-kappaB- and activator protein-1 (AP-1). Moreover, SF and LPS together are able to induce Nrf2 activation. We showed that SF, and also LPS by itself, are able to activate the cell's defence against oxidative an...Continue Reading

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangG H Posner
Jan 1, 1992·Brain Research. Brain Research Reviews·W E Thomas
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangP Talalay
Sep 21, 2000·Immunopharmacology·Y J JeonH M Kim
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A T Dinkova-KostovaP Talalay
Mar 28, 2001·Annals of the New York Academy of Sciences·U OttenS Rose-John
Jun 19, 2001·The Journal of Biological Chemistry·E HeissC Gerhäuser
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Henrik WilmsRalph Lucius
Apr 28, 2004·Free Radical Biology & Medicine·Anil K Jaiswal
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Jong-Min Lee, Jeffrey A Johnson
Jan 5, 2005·Nature Reviews. Immunology·Francisco González-Scarano, Julio Martín-García
Mar 15, 2005·The Journal of Biological Chemistry·Shivendra V SinghAnna Herman-Antosiewicz
Jan 13, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melinda C MyzakRoderick H Dashwood
Nov 14, 2006·Biochemical and Biophysical Research Communications·Rajesh K ThimmulappaShyam Biswal
Sep 21, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jing ZhaoPramod K Dash
Nov 7, 2007·Journal of Neural Transmission. Supplementum·C J WruckM E Götz
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·R Bryan Rock, Phillip K Peterson
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadia G InnamoratoAntonio Cuadrado
Aug 8, 2008·Journal of the Neurological Sciences·R E Gonsette
Oct 30, 2008·Journal of Neuropathology and Experimental Neurology·Lars-Ove BrandenburgRalph Lucius
Mar 24, 2009·Annual Review of Immunology·Richard M Ransohoff, V Hugh Perry
Mar 24, 2009·Annual Review of Immunology·Sivakumar Vallabhapurapu, Michael Karin
Apr 28, 2009·Frontiers in Neuroendocrinology·Markus Kipp, Cordian Beyer

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Jul 23, 2011·American Journal of Respiratory Cell and Molecular Biology·Milena HristovaAlbert van der Vliet
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Aug 27, 2013·Critical Reviews in Immunology·Alexander J Gill, Dennis L Kolson
Feb 19, 2011·Parkinson's Disease·Kemal Ugur TufekciKursad Genc
Aug 7, 2014·Calcified Tissue International·Sebastian LipprossChristoph Jan Wruck
Sep 21, 2011·Journal of Molecular Neuroscience : MN·Jon DangChristoph Jan Wruck
Sep 3, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·David J GraberBrent T Harris
Oct 24, 2012·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Federica SabatiniFabio L M Ricciardolo
Aug 7, 2013·Journal of Neuroimmunology·Sergey KalininDouglas L Feinstein
Sep 18, 2012·Biochemical and Biophysical Research Communications·Ju-Hee LeeSang-Youel Park
Dec 20, 2011·Mutation Research·Carmela FimognariPatrizia Hrelia
Jul 19, 2011·The Journal of Surgical Research·Lei MaoXianzhong Zhao
Feb 23, 2011·Biochemical and Biophysical Research Communications·Christina SvenssonTiit Land
Oct 7, 2014·Molecular Nutrition & Food Research·Danielle L FolkardRichard F Mithen
Oct 19, 2011·International Journal of Dermatology·Christoph J WruckThomas Pufe
Oct 22, 2013·The Journal of Pharmacy and Pharmacology·Sun Young ParkYoung-Whan Choi
Feb 24, 2010·Brain Research·Fabian BaertlingMarkus Kipp
Mar 6, 2015·Expert Opinion on Therapeutic Patents·Chien-Hsing Lee, Chung-Yi Chen
Dec 28, 2012·Pharmacology & Therapeutics·Scarlett Gillespie, Felicity N E Gavins
Jul 23, 2014·Drug Discovery Today·Maria H TrakaRichard F Mithen
Jan 5, 2012·Biochemical and Biophysical Research Communications·Mika YanagitaiTakumi Satoh
Apr 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rosaliana LibroEmanuela Mazzon
Sep 13, 2016·Antioxidants·Jamie L LimJack van Horssen
Jan 24, 2017·Journal of Neurochemistry·Sandra JansenLars-Ove Brandenburg
Sep 9, 2011·JPEN. Journal of Parenteral and Enteral Nutrition·Ryan T HurtMatt C Cave
Apr 1, 2019·Cancer Medicine·Shijia WuYongheng Bai
Mar 3, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Athanassios FragoulisChristoph Jan Wruck
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·N K ZenkovV O Tkachev
Apr 25, 2014·The Journal of Biological Chemistry·Antonio CuadradoIsabel Lastres-Becker

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