Sulfur bacteria in wastewater stabilization ponds periodically affected by the 'red-water' phenomenon.

Applied Microbiology and Biotechnology
Abdelaziz BelilaGerard Muyzer

Abstract

Several wastewater stabilization ponds (WSP) in Tunisia suffer periodically from the 'red-water' phenomenon due to blooming of purple sulfur bacteria, indicating that sulfur cycle is one of the main element cycles in these ponds. In this study, we investigated the microbial diversity of the El Menzeh WSP and focused in particular on the different functional groups of sulfur bacteria. For this purpose, we used denaturing gradient gel electrophoresis of PCR-amplified fragments of the 16S rRNA gene and of different functional genes involved in microbial sulfur metabolism (dsrB, aprA, and pufM). Analyses of the 16S rRNA revealed a relatively high microbial diversity where Proteobacteria, Chlorobi, Bacteroidetes, and Cyanobacteria constitute the major bacterial groups. The dsrB and aprA gene analysis revealed the presence of deltaproteobacterial sulfate-reducing bacteria (i.e., Desulfobacter and Desulfobulbus), while the analysis of 16S rRNA, aprA, and pufM genes assigned the sulfur-oxidizing bacteria community to the photosynthetic representatives belonging to the Chlorobi (green sulfur bacteria) and the Proteobacteria (purple sulfur and non sulfur bacteria) phyla. These results point on the diversity of the metabolic processes wit...Continue Reading

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Citations

Sep 17, 2013·Environmental Microbiology·Maryeimy Varon-LopezFernando Dini Andreote
Sep 30, 2016·Applied Microbiology and Biotechnology·Daniel Derrossi MeyerAdriano Brandelli
Feb 6, 2018·Environmental Technology·Lucy E ElandCesar R Mota Filho
Dec 23, 2017·Extremophiles : Life Under Extreme Conditions·Ekaterina I BurganskayaBoris B Kuznetsov

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Datasets Mentioned

BETA
HQ222639
HQ222674
HQ222675
HQ222729
HQ222613
HQ222638
HQ222730
HQ222810

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
ARB
Genesnap
ARB parsimony

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