Sulfur-deficient transfer ribonucleic acid in a cysteine-requiring, "relaxed" mutant of Escherichia coli.

Journal of Bacteriology
C L HarrisA L Cline

Abstract

A cysteine-requiring mutant of the parent strain Escherichia coli Hfr Cavalli (RC(rel), Met(-), lambda) has been isolated. The mutant was selected by using replica plating after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine. The mutation appears to be in the gene for sulfite reductase, since the mutant could utilize sulfide but not sulfite as a sulfur source. The mutant was found to be RC(rel) with respect to both methionine and cysteine. During cysteine starvation, transfer ribonucleic acid (tRNA) deficient in 4-thiouracil was produced, and in vivo studies indicate that this tRNA can accept sulfur groups to a greater extent than normal tRNA. Further, there were differences both in the rate and extent of amino acid acceptance between normal and sulfur-deficient tRNA. This suggests that thionucleotides are involved in at least one of the biological functions of the tRNA molecule.

References

Jan 14, 1969·Journal of Molecular Biology·C D Yegian, G S Stent
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·R S HaywardS B Weiss
Jan 14, 1969·Journal of Molecular Biology·M L Gefter, R L Russell
May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J A CarbonD S Jones
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R S Hayward, S B Weiss
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M N Lipsett, A Peterkofsky
Apr 1, 1967·Journal of Bacteriology·R C Bohinski, M F Mallette
Dec 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·G S STENT, S BRENNER
Mar 1, 1952·Journal of Bacteriology·J LEDERBERG, E M LEDERBERG

❮ Previous
Next ❯

Citations

Aug 31, 1981·Biochemical and Biophysical Research Communications·C L Harris, L Lui
Dec 7, 1977·Biochemical and Biophysical Research Communications·C L HarrisD Stewart
Mar 5, 1971·Biochemical and Biophysical Research Communications·Y Lalyre, E B Tirchener
Jan 1, 1989·Analytical Biochemistry·C L Harris, C J Kolanko
Jul 23, 1971·Science·D Söll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.