Sulfur Partitioning between Glutathione and Protein Synthesis Determines Plant Growth

Plant Physiology
Anna SpeiserRüdiger Hell

Abstract

Photoautotrophic organisms must efficiently allocate their resources between stress-response pathways and growth-promoting pathways to be successful in a constantly changing environment. In this study, we addressed the coordination of sulfur flux between the biosynthesis of the reactive oxygen species scavenger glutathione (GSH) and protein translation as one example of a central resource allocation switch. We crossed the Arabidopsis (Arabidopsis thaliana) GSH synthesis-depleted cadmium-sensitive cad2-1 mutant, which lacks glutamate cysteine (Cys) ligase, into the sulfite reductase sir1-1 mutant, which suffers from a significantly decreased flux of sulfur into Cys and, consequently, is retarded in growth. Surprisingly, depletion of GSH synthesis promoted the growth of the sir1-1 cad2-1 double mutant (s1c2) when compared with sir1-1 Determination of GSH levels and in vivo live-cell imaging of the reduction-oxidation-sensitive green fluorescent protein sensor demonstrated significant oxidation of the plastidic GSH redox potential in cad2-1 and s1c2 This oxidized GSH redox potential aligned with significant activation of plastid-localized sulfate reduction and a significantly higher flux of sulfur into proteins. The specific activ...Continue Reading

Citations

Feb 5, 2019·Journal of Experimental Botany·Yue WuJen Sheen
Mar 15, 2019·Journal of Experimental Botany·Kai Xun ChanFrank Van Breusegem
Mar 15, 2019·Journal of Experimental Botany·Wilena Telman, Karl-Josef Dietz
May 5, 2020·The Plant Journal : for Cell and Molecular Biology·Stefanie J Müller-SchüsseleAndreas J Meyer
Mar 3, 2019·Journal of Experimental Botany·Joseph M Jez
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Dec 2, 2020·International Journal of Molecular Sciences·Qian LiAn Yang
Mar 26, 2021·Ecotoxicology and Environmental Safety·Aditya Banerjee, Aryadeep Roychoudhury
Dec 15, 2020·Trends in Biochemical Sciences·Graham M Burkart, Federica Brandizzi
Jun 8, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Muhammad AqibMuhammad Munir Usmani
May 17, 2021·Journal of Experimental Botany·Björn Heinemann, Tatjana M Hildebrandt
Dec 11, 2021·Frontiers in Plant Science·Lingling DouHuaizhu Li

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