PMID: 9533768Apr 9, 1998Paper

Sulindac sulfide alters the expression of cyclin proteins in HT-29 colon adenocarcinoma cells

International Journal of Cancer. Journal International Du Cancer
L QiaoB Rigas

Abstract

Sulindac sulfide (SS), the active metabolite of the colon cancer chemopreventive compound sulindac, inhibits the proliferation of HT-29 colon cancer cells mainly by inducing cell quiescence. We determined by bivariate flow-cytometric analysis both the DNA and cyclin protein content of individual cells. Thus, we assessed in detail the expression of several cyclins during the cell-cycle phases and demonstrated that SS (i) decreases the expression of cyclins B1 and E and (ii) increases the expression of cyclins D1, D2 and D3, particularly in the G1 phase of the cell cycle. SS-induced apoptotic cells expressed both E- and D-type cyclins but not cyclin B1. The changes in cyclin expression combined with reduced catalytic activity of cyclin-dependent kinases could explain in molecular terms the anti-proliferative effect of SS on HT-29 colon cancer cells. These changes may contribute to the chemopreventive effect of sulindac.

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Citations

May 24, 2008·Carcinogenesis·Hector Guillen-AhlersVictoria A Ploplis
Nov 27, 2002·Cancer Investigation·Yaser RayyanBasil Rigas
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nuria A CallejasPaloma Martín-Sanz
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·E Fosslien
Jul 1, 2005·Digestive Diseases and Sciences·Hassan AshktorabDuane T Smoot
Sep 10, 1999·The Journal of Biological Chemistry·Y YamamotoR B Gaynor
Oct 19, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S GröschG Geisslinger
Aug 3, 2002·Journal of Neuropathology and Experimental Neurology·Jeroen J M HoozemansThomas Arendt
Jun 14, 2005·Biochemical Pharmacology·Khosrow Kashfi, Basil Rigas

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