Sulphate uptake and metabolism in the chrysomonad, monochrysis lutheri.

Archives of Microbiology
E M Deane, R W O'Brien

Abstract

The intracellular concentration of inorganic 35SO4 in Monochrysis lutheri cells exposed to 0.513 mM Na235SO4 for up to 6-hr remained constant at about 0.038 mM. The exchange rate of this 35SO4 with the external unlabelled sulphate was negligible compared to the rate of influx across the plasmalemma (0.032 mu moles/g cells/hr). The flux of free 35SO4 to organic 35S was 0.029 mu moles/g cells/hr. Assuming an internal electrical potential in the cells of -70 mV, this intracellular concentration of inorganic 35SO4 was well in excess of that obtainable by passive diffusion as calculated from the Nernst equation. These results indicate that sulphate is accumulated by an active mechanism rather than by facilitated diffusion. Sulphate uptake appears to occur via a carrier-mediated membrane transport system which conforms to Michaelis-Menten type saturation kinetics with a Km of 3.2 X 10(-5) M and Vmax of 7.9 X 10(-5) mu moles sulphate/hr/10(5) cells. Uptake was dependent on a source of energy since the metabolic inhibitor CCCP almost completely inhibited uptake under both light and dark conditions and DCMU caused a 50% decrease in uptake under light conditions. Under dark conditions, uptake remained at about 80% of that observed under ...Continue Reading

References

Jan 15, 1974·FEBS Letters·R G McCready, G A Din
Jun 1, 1966·Archives of Biochemistry and Biophysics·L A Yamamoto, I H Segel
Oct 15, 1972·FEBS Letters·S E. Springer, R E. Huber
Apr 1, 1962·Canadian Journal of Microbiology·R R GUILLARD, J H RYTHER
May 1, 1956·Plant Physiology·J E Leggett, E Epstein

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Citations

Jan 1, 1989·Archives of Microbiology·H Cypionka
Feb 20, 1979·Biochimica Et Biophysica Acta·G M RoomansG W Borst-Pauwels

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