PMID: 7525942Jul 15, 1994Paper

Sulphorhodamine-labelled cells in the neonatal rat spinal cord following chemically induced locomotor activity in vitro

The Journal of Physiology
O KjaerulffO Kiehn

Abstract

1. Sulphorhodamine 101, a fluorescent dye and newly identified activity marker, was used to localize potential spinal locomotor networks in the neonatal rat spinal cord. 2. Preparations of the spinal cord with one entire hindlimb attached or the spinal cord in isolation were kept in vitro. Spinal locomotor activity was maintained chemically with NMDA (5-7.5 microM), in combination with 5-HT (7.5-20 microM), for 4-4.5 h in the presence of 0.0001-0.0005% sulphorhodamine 101. Matched non-locomoting controls were exposed to the dye in the absence of transmitters for a comparable time. Transverse sections of the lumbar spinal cord (L1-L6) were screened for rhodamine emission using an epifluorescence microscope. 3. In hindlimb-attached locomoting preparations with intact dorsal roots, labelled cells were found on the leg side in the dorsal horn (mainly laminae II-IV), in the intermediate grey (lamina VI-VII) and around the central canal (lamina X). Dorsal rhizotomy was performed on the leg side, to prevent synaptic activity due to afferent inflow. This largely reduced the number of labelled cells in the dorsal horn and in the lateral part of the intermediate grey matter. A further reduction of labelling in these areas was seen after ...Continue Reading

Citations

Feb 3, 1999·Annals of the New York Academy of Sciences·O Kiehn, O Kjaerulff
Jan 5, 2002·The Journal of Comparative Neurology·Deborah M Antonino-GreenDavid S K Magnuson
Jun 15, 2007·Journal of Neurophysiology·Sabrina TazerartFrédéric Brocard
Apr 20, 2010·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Frédéric BrocardLaurent Vinay
Dec 31, 1997·The Journal of Comparative Neurology·Z Puskár, M Antal
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Oct 7, 2005·Journal of Neurophysiology·Jonathan R McDearmid, Pierre Drapeau
Jun 12, 2010·Annals of the New York Academy of Sciences·Andrea NistriAnujaianthi Kuzhandaivel
Mar 19, 2013·Brain Structure & Function·Huazheng LiangGeorge Paxinos
Feb 3, 1999·Annals of the New York Academy of Sciences·J R CazaletsF Clarac
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M RaastadO Kiehn
Jun 17, 2006·Annual Review of Neuroscience·Ole Kiehn
Sep 19, 2012·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Jonathan W MuiAri Berkowitz
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