Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5'-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing gamma-ECS, SO, or APR.

Journal of Experimental Botany
Ursula ScheererCornelia Herschbach

Abstract

Sulphate assimilation provides reduced sulphur for the synthesis of cysteine, methionine, and numerous other essential metabolites and secondary compounds. The key step in the pathway is the reduction of activated sulphate, adenosine 5'-phosphosulphate (APS), to sulphite catalysed by APS reductase (APR). In the present study, [(35)S]sulphur flux from external sulphate into glutathione (GSH) and proteins was analysed to check whether APR controls the flux through the sulphate assimilation pathway in poplar roots under some stress conditions and in transgenic poplars. (i) O-Acetylserine (OAS) induced APR activity and the sulphur flux into GSH. (ii) The herbicide Acetochlor induced APR activity and results in a decline of GSH. Thereby the sulphur flux into GSH or protein remained unaffected. (iii) Cd treatment increased APR activity without any changes in sulphur flux but lowered sulphate uptake. Several transgenic poplar plants that were manipulated in sulphur metabolism were also analysed. (i) Transgenic poplar plants that overexpressed the gamma-glutamylcysteine synthetase (gamma-ECS) gene, the enzyme catalysing the key step in GSH formation, showed an increase in sulphur flux into GSH and sulphate uptake when gamma-ECS was tar...Continue Reading

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Citations

Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Geoffrey E RaviliousJoseph M Jez
Jul 20, 2012·The Journal of Biological Chemistry·Geoffrey E Ravilious, Joseph M Jez
Mar 4, 2014·Nitric Oxide : Biology and Chemistry·Alexander Calderwood, Stanislav Kopriva
Jan 27, 2012·Metabolomics : Official Journal of the Metabolomic Society·Martin LehmannToshihiro Obata
May 8, 2013·Environmental Science and Pollution Research International·Blenda Machado-EstradaJosé S Rodríguez-Zavala
Sep 8, 2011·Plant, Cell & Environment·Dörte RandewigRobert Hänsch
Apr 9, 2013·The New Phytologist·Maria H TrakaRichard F Mithen
Nov 18, 2014·Frontiers in Plant Science·Anna Koprivova, Stanislav Kopriva
Jan 30, 2015·Frontiers in Plant Science·Silke C Weckopp, Stanislav Kopriva
Dec 6, 2014·Frontiers in Plant Science·Alexander CalderwoodStanislav Kopriva
Mar 4, 2014·FEBS Letters·Anna KoprivovaStanislav Kopriva
Dec 24, 2010·The Plant Journal : for Cell and Molecular Biology·Sarah G MugfordStanislav Kopriva
Mar 16, 2011·The Plant Journal : for Cell and Molecular Biology·Cintia G KawashimaStanislav Kopriva
Apr 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Sönke ScherzerRainer Hedrich
Jul 5, 2019·The Journal of Biological Chemistry·Süleyman GünalJonathan Wolf Mueller
Apr 26, 2019·Frontiers in Plant Science·Ursula ScheererCornelia Herschbach
Jan 9, 2019·Frontiers in Plant Science·Sladjana SamuilovHeinz Rennenberg
Dec 2, 2020·International Journal of Molecular Sciences·Qian LiAn Yang

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Methods Mentioned

BETA
transgenic
PCR
genetic modifications

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