PMID: 9422084Jan 9, 1998Paper

Summary report on the ISOBM TD-4 Workshop: analysis of 56 monoclonal antibodies against the MUC1 mucin. San Diego, Calif., November 17-23, 1996

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
M R PriceJ Hilgers

Abstract

Sixteen research groups participated in the ISOBM TD-4 Workshop in which the reactivity and specificity of 56 monoclonal antibodies against the MUC1 mucin was investigated using a diverse panel of target antigens and MUC1 mucin-related synthetic peptides and glycopeptides. The majority of antibodies (34/56) defined epitopes located within the 20-amino acid tandem repeat sequence of the MUC1 mucin protein core. Of the remaining 22 antibodies, there was evidence for the involvement of carbohydrate residues in the epitopes for 16 antibodies. There was no obvious relationship between the type of immunogen and the specificity of each antibody. Synthetic peptides and glycopeptides were analyzed for their reactivity with each antibody either by assay of direct binding (e.g. by ELISA or BiaCore) or by determining the capacity of synthetic ligands to inhibit antibody binding interactions. There was good concordance between the research groups in identifying antibodies reactive with peptide epitopes within the MUC1 protein core. Epitope mapping tests were performed using the Pepscan analysis for antibody reactivity against overlapping synthetic peptides, and results were largely consistent between research groups. The dominant feature of...Continue Reading

Citations

Mar 1, 2007·Indian Journal of Clinical Biochemistry : IJCB·K ThriveniGirija Ramaswamy
Feb 18, 2006·Cancer Immunology, Immunotherapy : CII·Antje DanielczykSteffen Goletz
Sep 9, 2010·Cancer Immunology, Immunotherapy : CII·Julia PinkhasovPinku Mukherjee
Jun 14, 2012·Cancer Immunology, Immunotherapy : CII·Pearline Zhaoying TeoJoseph A Mollick
Nov 22, 2011·Glycoconjugate Journal·Emma ArigiSteven B Levery
Oct 20, 2009·Targeted Oncology·Gairin DanceyTim Meyer
Sep 16, 2011·Current Treatment Options in Oncology·Vandana G Abramson, Ingrid A Mayer
Apr 3, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rafael MolinaMartin Velasco
Sep 6, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mohamed ElfagiehAbdelbaset Buhmeida
Oct 26, 1999·Cancer Letters·M StimpflR Zeillinger
Mar 20, 2001·Critical Reviews in Oncology/hematology·C M Richman, S J DeNardo
Aug 24, 2010·Journal of Proteome Research·Ola BlixtKnud J Jensen
Oct 5, 2010·Journal of Proteome Research·Stjepan K KracunOla Blixt
Aug 29, 2009·Journal of Proteome Research·Yi-Mi WuBrian B Haab
Nov 19, 2003·Breast Cancer Research and Treatment·María V CroceAmada Segal-Eiras
Feb 13, 2002·Hybridoma and Hybridomics·W QiR Madiyalakan
Sep 5, 2003·Hybridoma and Hybridomics·M PaknejadM Rajabi Bazl
Jul 7, 2011·Cancer Biotherapy & Radiopharmaceuticals·Huguette Albrecht, Kermit L Carraway
Mar 11, 2000·Molecular Biology of the Cell·Y AltschulerR P Hughey
Dec 24, 2008·Nucleic Acids Research·Cátia S M FerreiraJean Gariépy
Feb 21, 2006·Biochemistry. Biokhimii︠a︡·S J Golubović, Z T Bojić-Trbojević
Oct 13, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·C S M FerreiraS Missailidis
Dec 18, 2012·BMC Cancer·Verena EngelstaedterUdo Jeschke
Jan 26, 2011·PloS One·Holger ThieStefan Dübel
Jul 5, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Danni L Meany, Daniel W Chan
Jun 25, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Marie-France PichonNatacha Charlier-Bret
Feb 13, 2013·Molecular & Cellular Proteomics : MCP·Kevin Chandler, Radoslav Goldman
Nov 24, 2005·Immunological Investigations·Fatemeh RahbarizadehMajid Sadeghizadeh
Sep 8, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michael J DuffyEnda W McDermott
Feb 10, 2009·Veterinary Immunology and Immunopathology·Ezequiel LacunzaMaría V Croce
Oct 14, 2008·Nuclear Medicine and Biology·Mojtaba SaloutiMohammad Javad Rasaee
Jun 10, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Andreas KuemmelRainer Wiewrodt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
A BlockzjilO Nilsson
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
K RyukoJ Hilgers
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
U KarstenSteffen Goletz
© 2021 Meta ULC. All rights reserved