SUMO mediating fusion expression of antimicrobial peptide CM4 from two joined genes in Escherichia coli.

Current Microbiology
Jian Feng LiShuang Quan Zhang

Abstract

Antibacterial peptide CM4 (ABP-CM4) is a small cationic peptide with broad-spectrum activities against bacteria, fungi, and tumor cells, which may possibly be used as an antimicrobial agent. To improve the expression level of CM4 in Escherichia coli, two tandem repeats of CM4 genes were cloned into the vector pSUMO to construct an expression vector pSUMO-2CM4. The fusion protein SUMO-2CM4, purified by Ni(2+)-chelating chromatography, was cleaved by hydroxylamine hydrochloride to release recombinant CM4. After the cleaved sample was re-applied to a Ni-IDA column, finally, about 48 mg recombinant CM4 was obtained from 1 L bacterial culture with no less than 96% purity, which was the highest yield of CM4 reported so far.

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Citations

Sep 25, 2012·Applied Biochemistry and Biotechnology·Min Keun KimHan Dae Yun
Oct 2, 2012·Peptides·Nádia Skorupa ParachinOctávio Luiz Franco
Nov 4, 2016·Preparative Biochemistry & Biotechnology·Siwei XiaoYuanxing Zhang
Jan 11, 2017·Scientific Reports·Shengyue JiHongfu Zhang
Aug 19, 2018·Applied Microbiology and Biotechnology·Baode SunChun-Xia Zhao
May 2, 2020·Expert Review of Clinical Pharmacology·Kamila Botelho Sampaio de OliveiraSimoni Campos Dias
Jan 30, 2020·Applied Microbiology and Biotechnology·Mi-Ran Ki, Seung Pil Pack

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