SUMO polymeric chains are involved in nuclear foci formation and chromatin organization in Trypanosoma brucei procyclic forms

PloS One
Paula Ana IribarrenV E Alvarez

Abstract

SUMOylation is a post-translational modification conserved in eukaryotic organisms that involves the covalent attachment of the small ubiquitin-like protein SUMO to internal lysine residues in target proteins. This tag usually alters the interaction surface of the modified protein and can be translated into changes in its biological activity, stability or subcellular localization, among other possible outputs. SUMO can be attached as a single moiety or as SUMO polymers in case there are internal acceptor sites in SUMO itself. These chains have been shown to be important for proteasomal degradation as well as for the formation of subnuclear structures such as the synaptonemal complex in Saccharomyces cerevisiae or promyelocytic leukemia nuclear bodies in mammals. In this work, we have examined SUMO chain formation in the protozoan parasite Trypanosoma brucei. Using a recently developed bacterial strain engineered to produce SUMOylated proteins we confirmed the ability of TbSUMO to form polymers and determined the type of linkage using site-directed mutational analysis. By generating transgenic procyclic parasites unable to form chains we demonstrated that although not essential for normal growth, SUMO polymerization determines t...Continue Reading

References

Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Jul 14, 2001·The Journal of Biological Chemistry·M H TathamR T Hay
Jul 4, 2002·The Journal of Eukaryotic Microbiology·D Pérez-MorgaE Pays
Aug 28, 2003·The Journal of Biological Chemistry·Gwendolyn R BylebylErica S Johnson
Sep 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jing SongYuan Chen
Dec 6, 2005·Developmental Cell·Karim NacerddineAnne Dejean
Dec 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ville HietakangasLea Sistonen
Mar 10, 2006·The Journal of Biological Chemistry·Christina-Maria HeckerIvan Dikic
Oct 13, 2006·The EMBO Journal·Shen-Hsi YangAndrew D Sharrocks
Mar 3, 2007·Bioinformatics·Markus BrameierRobert M MacCallum
Apr 15, 2008·Nature Cell Biology·Michael H TathamRonald T Hay
Nov 11, 2008·Molecular Cell·Helle D Ulrich
Oct 22, 2009·Nucleic Acids Research·Martin AslettHaiming Wang
Jan 16, 2010·Biochemical Society Transactions·Alfred C O Vertegaal
Sep 25, 2010·Nucleic Acids Research·Samson O ObadoJohn M Kelly
Nov 26, 2010·Nature Reviews. Molecular Cell Biology·Jaclyn R Gareau, Christopher D Lima
Jan 22, 2011·EMBO Reports·Roland BrudererRonald T Hay
Aug 13, 2011·Molecular & Cellular Proteomics : MCP·Julio C BayonaVanina E Alvarez
Nov 20, 2012·Nucleic Acids Research·Christian J A SigristIoannis Xenarios
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Christopher M HickeyMark Hochstrasser
Apr 3, 2013·The Journal of Cell Biology·Tharan SrikumarBrian Raught
Jun 12, 2013·Annual Review of Biochemistry·Annette Flotho, Frauke Melchior
Oct 18, 2013·PeerJ·Cornelia Andrea KleinChristine Clayton
Feb 4, 2014·Current Biology : CB·Lisa M D'Ambrosio, Brigitte D Lavoie
Feb 11, 2014·Current Biology : CB·Ding HeSandra L Baldauf
May 2, 2014·Science Signaling·Triin TammsaluRonald T Hay
Sep 15, 2014·Nature Structural & Molecular Biology·Ivo A HendriksAlfred C O Vertegaal
Jul 5, 2015·Bioinformatics·Guillaume BeauclairAlessia Zamborlini
Aug 11, 2015·PloS One·Paula Ana IribarrenVanina Eder Alvarez
Nov 26, 2015·The Journal of Cell Biology·Wing-Kit LeungHideo Tsubouchi
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
Jul 21, 2016·Nature Reviews. Molecular Cell Biology·Ivo A Hendriks, Alfred C O Vertegaal
Nov 18, 2016·Molecular & Cellular Proteomics : MCP·Teresa Cristina Leandro de JesusJulia Pinheiro Chagas da Cunha

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Citations

Oct 8, 2020·Biomolecules·Maryia Karpiyevich, Katerina Artavanis-Tsakonas

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Methods Mentioned

BETA
transgenic
pull-down
transfection
PCR
pull down

Software Mentioned

SUMO
Image Studio
Image
SUMOAMVR
Studio
GPS
Tritrypdb Analyze Results
NucPred
ImageJ
IUPRED

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