Sumoylated NHR-25/NR5A regulates cell fate during C. elegans vulval development

PLoS Genetics
Jordan D WardKeith R Yamamoto

Abstract

Individual metazoan transcription factors (TFs) regulate distinct sets of genes depending on cell type and developmental or physiological context. The precise mechanisms by which regulatory information from ligands, genomic sequence elements, co-factors, and post-translational modifications are integrated by TFs remain challenging questions. Here, we examine how a single regulatory input, sumoylation, differentially modulates the activity of a conserved C. elegans nuclear hormone receptor, NHR-25, in different cell types. Through a combination of yeast two-hybrid analysis and in vitro biochemistry we identified the single C. elegans SUMO (SMO-1) as an NHR-25 interacting protein, and showed that NHR-25 is sumoylated on at least four lysines. Some of the sumoylation acceptor sites are in common with those of the NHR-25 mammalian orthologs SF-1 and LRH-1, demonstrating that sumoylation has been strongly conserved within the NR5A family. We showed that NHR-25 bound canonical SF-1 binding sequences to regulate transcription, and that NHR-25 activity was enhanced in vivo upon loss of sumoylation. Knockdown of smo-1 mimicked NHR-25 overexpression with respect to maintenance of the 3° cell fate in vulval precursor cells (VPCs) during d...Continue Reading

References

Mar 1, 1974·Comparative and General Pharmacology·R Pertel, S H Wilson
Sep 6, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M AsahinaS Hirose
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sam HolmstromJorge A Iñiguez-Lluhi
May 8, 2004·Trends in Cell Biology·Elisabeth FayardKristina Schoonjans
Jun 12, 2004·The Journal of Biological Chemistry·Wei-Yi ChenBon-Chu Chung
Feb 17, 2005·Molecular and Cellular Biology·Martin B LeeHolly A Ingraham
Jun 1, 2005·Molecular and Cellular Biology·Angeliki Chalkiadaki, Iannis Talianidis
Jun 18, 2005·Molecular Endocrinology·Stephanie SentisLaura Corbo
Jun 29, 2005·Journal of Molecular Evolution·Marc Robinson-RechaviAnn Sluder
Aug 27, 2005·PLoS Biology·Alison R FrandGary Ruvkun
Jun 17, 2006·Cell·Bart DeplanckeAlbertha J M Walhout
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Paul W Sternberg
Apr 15, 2008·Nature Cell Biology·Michael H TathamRonald T Hay
Oct 8, 2008·Molecular and Cellular Biology·Lioudmila A CampbellHolly A Ingraham
Oct 28, 2008·Nature Genetics·Christian Frøkjaer-JensenErik M Jorgensen
Dec 2, 2008·Biochemical and Biophysical Research Communications·Eun Ju HwangKeun Il Kim
Dec 25, 2008·Methods in Molecular Biology·Mary B Kroetz, Mark Hochstrasser
Dec 25, 2008·Methods in Molecular Biology·Ali A Yunus, Christopher D Lima
Dec 25, 2008·Methods in Molecular Biology·David Reverter, Christopher D Lima

❮ Previous
Next ❯

Citations

Apr 25, 2017·Nature Cell Biology·Chih-Chun Janet Lin, Meng C Wang
Jul 2, 2014·Biochimica Et Biophysica Acta·Marta Kostrouchova, Zdenek Kostrouch
Nov 7, 2019·Journal of Clinical Medicine·Sajad DehnaviAmirhossein Sahebkar
May 7, 2020·Open Biology·Ana TalamilloRosa Barrio
Dec 7, 2018·Genetics·Gabriela C MonsalveJordan D Ward
Nov 16, 2019·G3 : Genes - Genomes - Genetics·Michael A Q MartinezDavid Q Matus
Mar 8, 2021·Genetics·Guinevere E AshleyJordan D Ward
Jul 21, 2020·Psychoneuroendocrinology·Hsiao-Yun LinDah-Yuu Lu
Apr 16, 2015·G3 : Genes - Genomes - Genetics·Angenee C Milton, Peter G Okkema
Apr 17, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Yu SunKristina Schoonjans

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
sumoylation
Y2H
immunoprecipitation
three-hybrid
one-hybrid
electrophoretic mobility shift
Y1H
transgenic
PCR

Software Mentioned

SUMOsp2
SUMOplot
BLAST

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.