Sumoylation controls the timing of Tup1-mediated transcriptional deactivation

Nature Communications
Chong Han NgJames L Manley

Abstract

The small ubiquitin-like modifier (SUMO) is implicated in various cellular activities, including transcriptional regulation. We previously showed that the yeast activator Gcn4 becomes sumoylated during activation, facilitating its eventual promoter eviction and transcriptional shut off. Here we show that the corepressor Tup1 is sumoylated, at two specific lysines, under various stress conditions. Mutation of these sites has no effect on Tup1 recruitment or RNAP II promoter occupancy immediately following induction. However, Tup1 levels subsequently decrease, while RNAP II and transcription increase in Tup1 mutant cells. Consistent with this, a Tup1 mutant displaying increased sumoylation led to reduced transcription. We also show that coordinated sumoylation of Gcn4 and Tup1 enhances Gcn4 promoter eviction and that multiple Tup1-interacting proteins become sumoylated after stress. Together, our studies provide evidence that coordinated sumoylation of Gcn4, Tup1 and likely other factors dampens activated transcription by stabilizing Tup1 binding and stimulating Gcn4 and RNAP II removal.

References

Feb 21, 1992·Cell·C A KeleherA D Johnson
Dec 1, 1996·Molecular and Cellular Biology·U S VaranasiR J Trumbly
Apr 25, 1997·The Journal of Biological Chemistry·M J ReddA D Johnson
Jun 29, 2000·Trends in Biochemical Sciences·R L Smith, A D Johnson
Apr 1, 2003·Molecular and Cellular Biology·Mark J SwansonAlan G Hinnebusch
Aug 28, 2003·The Journal of Biological Chemistry·Gwendolyn R BylebylErica S Johnson
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Aug 25, 2004·The Journal of Biological Chemistry·James A WohlschlegelJohn R Yates
Nov 16, 2004·Molecular & Cellular Proteomics : MCP·Carilee DenisonSteven P Gygi
Dec 15, 2004·Molecular & Cellular Proteomics : MCP·Dennis D Wykoff, Erin K O'Shea
Jun 21, 2005·Molecular and Cellular Biology·Chhabi K GovindAlan G Hinnebusch
Aug 13, 2005·Current Opinion in Genetics & Development·Grace Gill
Nov 30, 2005·Molecular and Cellular Biology·Soon-Ja KimAlan G Hinnebusch
Jan 18, 2006·Biochemistry·David E SternerShelley L Berger
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Tania M Malavé, Sharon Y R Dent
Dec 23, 2008·Biochemical and Biophysical Research Communications·Jang-Won AhnCheol Yong Choi
Jul 21, 2009·Biochimica Et Biophysica Acta·Mario Garcia-Dominguez, Jose C Reyes
May 28, 2010·Genes & Development·Emanuel RosoninaJames L Manley
Sep 4, 2012·Nucleic Acids Research·Hui-wen LiuJeffrey D Parvin
Jan 19, 2013·Developmental Cell·Caelin Cubeñas-Potts, Michael J Matunis
Jun 12, 2013·Annual Review of Biochemistry·Annette Flotho, Frauke Melchior

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Citations

Sep 12, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pierre ChymkowitchJorrit M Enserink
Feb 15, 2019·PLoS Genetics·Veroni S Sri TheivakadadchamEmanuel Rosonina
May 10, 2020·Nature Communications·Janis Tam, Folkert J van Werven
Jan 31, 2020·Frontiers in Cell and Developmental Biology·Jan Keiten-SchmitzStefan Müller
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mathias BoulangerGuillaume Bossis
Jan 28, 2021·ELife·Nikhil R BhagwatNeil Hunter
Dec 10, 2016·Analytical Chemistry·Thillai V SekarRamasamy Paulmurugan

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Methods Mentioned

BETA
sumoylation
ChIP
co-IP
PCR
Immunoprecipitation

Software Mentioned

SUMOsp

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