Sumoylation of CCAAT/enhancer-binding protein α is implicated in hematopoietic stem/progenitor cell development through regulating runx1 in zebrafish

Scientific Reports
Hao YuanJun Zhu

Abstract

The small ubiquitin-related modifier (SUMO) participates in various cellular processes, including maintenance of genome integrity, nuclear transport, transcription and signal transduction. However, the biological function of sumoylation in hematopoiesis has not been fully explored. We show here that definitive hematopoietic stem/progenitor cells (HSPCs) are depleted in SUMO-deficient zebrafish embryos. Impairment of sumoylation attenuates HSPC generation and proliferation. The hyposumoylation triggered HSPC defects are CCAAT/enhancer-binding protein α (C/ebpα) dependent. Critically, a SUMO-C/ebpα fusion rescues the defective hematopoiesis in SUMO-deficient embryos, at least in part through restored runx1 expression. While C/ebpα-dependent transcription is involved in myeloid differentiation, our studies here reveal that C/ebpα sumoylation is essential for HSPC development during definitive hematopoiesis.

References

Jul 1, 1988·Genes & Development·W H LandschulzS L McKnight
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Sep 1, 1996·Molecular and Cellular Biology·M OelgeschlägerA D Friedman
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·A M FordT Enver
Dec 13, 1996·Cell·J S Eisen
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·D E ZhangD G Tenen
Dec 31, 1997·Molecular and Cellular Biology·N A TimchenkoG J Darlington
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A L Kurtzman, N Schechter
Jul 12, 2002·Developmental Cell·Toshihiro MiyamotoKoichi Akashi
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stéphane BerghmansA Thomas Look
Dec 6, 2005·Developmental Cell·Karim NacerddineAnne Dejean
Oct 13, 2006·Molecular Biology of the Cell·Matthias Nowak, Matthias Hammerschmidt
May 29, 2007·PLoS Genetics·Mara E RobuStephen C Ekker
Sep 19, 2007·Blood·Dorothée Le GuyaderPhilippe Herbomel
Jan 10, 2008·Blood·Duncan Carradice, Graham J Lieschke
Feb 26, 2008·Cell·Stuart H Orkin, Leonard I Zon
Feb 25, 2009·Journal of Cellular Physiology·Alan D Friedman
Feb 16, 2010·Nature·Jean-Charles BoissetCatherine Robin
Aug 17, 2010·The International Journal of Developmental Biology·Elizabeth J Paik, Leonard I Zon
Aug 9, 2011·Nature Biotechnology·Peng HuangBo Zhang
May 30, 2013·Development·Madhumita Jagannathan-Bogdan, Leonard I Zon
Jun 12, 2013·Annual Review of Biochemistry·Annette Flotho, Frauke Melchior
Aug 7, 2013·Blood Cells, Molecules & Diseases·Sonja BoatmanLeonard I Zon

❮ Previous
Next ❯

Citations

Nov 5, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yimei DaiWenqing Zhang
Nov 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Joshua D BernstockWulf Paschen
Feb 25, 2017·Frontiers in Plant Science·Kamlesh K MeenaParamjit S Minhas

❮ Previous
Next ❯

Methods Mentioned

BETA
sumoylation
transgenic
Transfection
PCR
electrophoresis

Software Mentioned

ASW
TAL Effector Nucleotide Targeter
FV10

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.