Sumoylation regulates Kap114-mediated nuclear transport.

The EMBO Journal
Ute RothenbuschGabriel Schlenstedt

Abstract

The nuclear import receptor Kap114 carries transcription factors and other cargos across nuclear pores into the nucleus. Here we show that yeast Kap114 is modified by SUMO (small ubiquitin-related modifier) and that sumoylation is required for Kap114-mediated nuclear import. Among the four known SUMO-specific E3 ligases in yeast, Mms21 is the preferred E3 enzyme responsible for the covalent attachment of SUMO to the Kap114 protein. Kap114 is sumoylated on lysine residue 909, which is part of a ΨKxD/E sumoylation consensus motif. Kap114 containing a lysine-to-arginine point mutation at position 909 mislocalizes to the nucleus and is defective in promoting nuclear import. Similarly, mutants defective in sumoylation or desumoylation specifically accumulate Kap114 in the nucleus and are blocked in import of Kap114 cargos. Ran-GTP is not sufficient to disassemble Kap114/cargo complexes, which necessitates additional cargo release mechanisms in the nucleus. Remarkably, sumoylation of Kap114 greatly stimulates cargo dissociation in vitro. We propose that sumoylation occurs at the site of Kap114 cargo function and that SUMO is a cargo release factor involved in intranuclear targeting.

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 1, 1972·Molecular & General Genetics : MGG·G MazzaM Polsinelli
Jul 28, 1995·The Journal of Biological Chemistry·R J DohmenA Varshavsky
Oct 27, 1997·The Journal of Biological Chemistry·E S Johnson, G Blobel
Feb 7, 1998·The Journal of Cell Biology·L F PembertonG Blobel
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MorehouseS Buratowski
Mar 14, 2000·Molecular and Cellular Biology·S J Li, M Hochstrasser
Jul 25, 2000·Molecular & General Genetics : MGG·I SchwienhorstR J Dohmen
Oct 25, 2000·Molecular and Cellular Biology·J SolsbacherG Schlenstedt
Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Apr 20, 2001·The Journal of Cell Biology·N MosammaparastL F Pemberton
Oct 24, 2002·The Journal of Biological Chemistry·Katrin StadeThomas Sommer
Nov 29, 2002·The EMBO Journal·Nima MosammaparastLucy F Pemberton
Aug 28, 2003·The Journal of Biological Chemistry·Gwendolyn R BylebylErica S Johnson
Sep 25, 2003·Cellular and Molecular Life Sciences : CMLS·H Fried, U Kutay
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Feb 1, 2005·European Journal of Cell Biology·Markus GreinerGabriel Schlenstedt
Feb 11, 2005·Traffic·Lucy F Pemberton, Bryce M Paschal
Feb 22, 2005·Nature Structural & Molecular Biology·Andrea PichlerTitia K Sixma
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiaolan Zhao, Günter Blobel
Apr 6, 2005·Molecular Cell·Ronald T Hay
May 13, 2005·Molecular Biology of the Cell·Jennifer L HodgesLucy F Pemberton
Apr 4, 2006·Molecular and Cellular Biology·Stefanie CaesarGabriel Schlenstedt
Nov 2, 2006·Journal of Cell Science·Alison ReindleErica S Johnson
Apr 4, 2007·The Journal of Cell Biology·Taras MakhnevychRichard W Wozniak
May 18, 2007·Annual Review of Biochemistry·Atlanta CookElena Conti
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Mar 21, 2008·Molecular & Cellular Proteomics : MCP·Yu QuanTao Tao
May 14, 2010·The Biochemical Journal·Kevin A Wilkinson, Jeremy M Henley
Jul 22, 2010·Molecular and Cellular Biology·Yaroslav SydorskyyBrian Raught

❮ Previous
Next ❯

Citations

Jun 12, 2013·Annual Review of Biochemistry·Annette Flotho, Frauke Melchior
Jan 28, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marco P Licciardello, Stefan Kubicek
May 9, 2012·The EMBO Journal·Andreas Werner, Frauke Melchior
Apr 23, 2014·Biochimica Et Biophysica Acta·Cecilia V VranychAndrea S Rópolo
Apr 22, 2015·ELife·Ute FischerVikram Govind Panse
Feb 7, 2017·PLoS Genetics·Heather A NewmanMichael J Matunis
Mar 7, 2019·Journal of Cell Science·Hanne FolzCatherine Dargemont
Sep 12, 2018·Nature Communications·Sabina SchützVikram Govind Panse
Nov 23, 2016·Current Genetics·Lakshmi MahendrawadaShikha Laloraya
Aug 8, 2015·The Journal of Biological Chemistry·Volkan SakinFrauke Melchior
Apr 27, 2018·Nature Communications·Jérôme O RouvièreBenoit Palancade
Jul 19, 2019·Molecular and Cellular Endocrinology·Cyril S Anyetei-AnumLizabeth A Allison
Jun 3, 2021·International Journal of Molecular Sciences·Mitzi Díaz-HernándezEsther Orozco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.