Sunflower metallothionein family characterisation. Study of the Zn(II)- and Cd(II)-binding abilities of the HaMT1 and HaMT2 isoforms

Journal of Inorganic Biochemistry
M TomasRoger Bofill

Abstract

Plant metallothioneins (MTs) constitute a family of small Cys-rich proteins capable of coordinating metal ions, significantly differing from microbial and animal MTs. They are divided into four subfamilies depending on the Cys pattern in their sequence. In this work, the MT system of the sunflower plant (Helianthus annuus) has been defined, with ten genes coding for MTs (HaMT) belonging to the four plant MT subfamilies; three HaMT1, four HaMT2, one HaMT3 and two HaMT4 isoforms. The gene expression pattern and capacity to confer metal resistance to yeast cells have been analysed for at least one member of each subfamily. The divalent metal ion-binding abilities of HaMT1-2 and HaMT2-1 (the isoforms encoded by the most abundantly expressed HaMT1 and HaMT2 isogenes) have been characterised, as HaMT3 and HaMT4 were previously studied. Those isoforms constitute an optimum material to study the effect of Cys number variability on their coordination abilities, as they exhibit additional Cys residues regarding the canonical Cys pattern of each subfamily. Our results show that the variation in the number of Cys does not drastically modify their M(II)-binding abilities, but instead modulates the degree of heterogeneity of the correspondin...Continue Reading

References

Aug 25, 2001·Insect Biochemistry and Molecular Biology·P J HensbergenN M van Straalen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Nov 1, 2009·Metallomics : Integrated Biometal Science·Xiaoqiong Wan, Eva Freisinger
May 23, 2013·Metallomics : Integrated Biometal Science·Oksana I LeszczyszynClaudia A Blindauer
Jul 10, 2013·Metallomics : Integrated Biometal Science·Estevão A PerozaEva Freisinger

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Citations

Jun 16, 2016·Biochemistry. Biokhimii︠a︡·Lan-Song LiXu-Guang Liu
Sep 21, 2017·Frontiers in Plant Science·Md Kamrul HasanZhen-Yu Qi
Dec 9, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hasan T Imam, Claudia A Blindauer

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