Sunflower Plants as Bioindicators of Environmental Pollution with Lead (II) Ions.

Sensors
Olga KrystofovaRene Kizek

Abstract

In this study, the influence of lead (II) ions on sunflower growth and biochemistry was investigated from various points of view. Sunflower plants were treated with 0, 10, 50, 100 and/or 500 μM Pb-EDTA for eight days. We observed alterations in growth in all experimental groups compared with non-treated control plants. Further we determined total content of proteins by a Bradford protein assay. By the eighth day of the experiment, total protein contents in all treated plants were much lower compared to control. Particularly noticeable was the loss of approx. 8 μg/mL or 15 μg/mL in shoots or roots of plants treated with 100 mM Pb-EDTA. We also focused our attention on the activity of alanine transaminase (ALT), aspartate transaminase (AST) and urease. Activity of the enzymes increased with increasing length of the treatment and applied concentration of lead (II) ions. This increase corresponds well with a higher metabolic activity of treated plants. Contents of cysteine, reduced glutathione (GSH), oxidized glutathione (GSSG) and phytochelatin 2 (PC2) were determined by high performance liquid chromatography with electrochemical detection. Cysteine content declined in roots of plants with the increasing time of treatment of plant...Continue Reading

References

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Aug 15, 2007·Talanta·Maria das Graças Andrade KornSérgio Luis Costa Ferreira

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Citations

Mar 8, 2013·Chemosphere·Hazrat AliMuhammad Anwar Sajad
Dec 4, 2012·International Journal of Environmental Research and Public Health·Jiri SkladankaAdam Nawrath

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Methods Mentioned

BETA
biosensors
X-ray
nuclear magnetic resonance
protein assay

Software Mentioned

BS
200

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