Super-accuracy and super-resolution getting around the diffraction limit

Methods in Enzymology
Erdal ToprakPaul R Selvin

Abstract

In many research areas such as biology, biochemistry, and biophysics, measuring distances or identifying and counting objects can be of great importance. To do this, researchers often need complicated and expensive tools in order to have accurate measurements. In addition, these measurements are often done under nonphysiological settings. X-ray diffraction, for example, gets Angstrom-level structures, but it requires crystallizing a biological specimen. Electron microscopy (EM) has about 10A resolution, but often requires frozen (liquid nitrogen) samples. Optical microscopy, while coming closest to physiologically relevant conditions, has been limited by the minimum distances to be measured, typically about the diffraction limit, or approximately 200 nm. However, most biological molecules are <5-10nm in diameter, and getting molecular details requires imaging at this scale. In this chapter, we will describe some of the experimental approaches, from our lab and others, that push the limits of localization accuracy and optical resolution in fluorescence microscopy.

Citations

Mar 1, 2012·The Journal of Biological Chemistry·Jessica N Mazerik, Matthew J Tyska
Feb 23, 2013·International Journal of Molecular Sciences·Carina MonicoFrancesco S Pavone
Jul 4, 2012·Journal of Pathology Informatics·Yu ChenLiron Pantanowitz
Jul 7, 2011·International Journal of Molecular Sciences·Sheng-Wen Chiu, Mark C Leake
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Dec 25, 2010·Journal of the American Chemical Society·Ibrahim YildizFrançisco M Raymo

❮ Previous
Next ❯

Related Concepts