Super-Chelators for Advanced Protein Labeling in Living Cells

Angewandte Chemie
Karl GatterdamRobert Tampé

Abstract

Live-cell labeling, super-resolution microscopy, single-molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of protein function is essential to derive unbiased insights into cellular processes. Herein, we define a new class of hexavalent N-nitrilotriacetic acid (hexaNTA) chelators, displaying the highest affinity and stability of all NTA-based small interaction pairs described so far. Coupled to bright organic fluorophores with fine-tuned photophysical properties, the super-chelator probes were delivered into human cells by chemically gated nanopores. These super-chelators permit kinetic profiling, multiplexed labeling of His6 - and His12 -tagged proteins as well as single-molecule-based super-resolution imaging.

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Citations

Mar 29, 2019·Angewandte Chemie·Ralph Wieneke, Robert Tampé
Mar 22, 2020·Communications Biology·Stefan BrüchertRobert Tampé
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ohad SussDavid Margulies
Aug 8, 2018·Journal of the American Chemical Society·Viktorija GlembockyteGonzalo Cosa
Dec 21, 2018·The Journal of Physical Chemistry. a·Anuja SinghAshok Kumar Mishra

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