Abstract
Dicer-2 is a ribonuclease involved in the insect RNAi pathway. On attempting to knockdown Dicer-2 expression in the insect Blattella germanica by RNAi, we found that treatment with Dicer-2 dsRNA upregulated the targeted mRNA. This unexpected result was also observed after treating with a nucleopolyhedrovirus dsRNA. Experiments with this alien dsRNA showed an all-or-none response with a threshold for inducing Dicer-2 upregulation between 0.4 and 0.04 μg in terms of dsRNA concentration and between 50 and 20 bp in terms of dsRNA length. The response seems specific of dsRNA given that equivalent experiments carried out with dsDNA did not affect Dicer-2 expression. In insects, Dicer-2 is postulated to be a sensor of viral infections and a key antiviral defense element. The upregulation of Dicer-2 expression after dsRNA administration fits well with this sensor role, and the occurrence of this mechanism in B. germanica, a phylogenetically basal insect, suggests that sensing alien RNAs might be an ancestral function of Dicer-2 proteins.
References
Oct 20, 2001·Genes & Development·R F KettingR H Plasterk
Apr 25, 2002·Nucleic Acids Research·Michael W PfafflLeo Dempfle
Apr 7, 2004·Cell·Young Sik LeeRichard W Carthew
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Mar 24, 2006·Nature Immunology·Delphine Galiana-ArnouxJean-Luc Imler
May 13, 2006·The Journal of General Virology·D V MukhaC Schal
Oct 28, 2008·Nature Immunology·Safia DeddoucheJean-Luc Imler
Mar 12, 2009·Molecular Biology and Evolution·Danielle de JongBernd Schierwater
Dec 8, 2009·Annual Review of Entomology·Xavier Bellés
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eva Gomez-Orte, Xavier Belles
Feb 25, 2010·Molecular Biology and Evolution·Stéphanie Jaubert-PossamaiDenis Tagu
Aug 14, 2010·Nature Reviews. Immunology·Shou-Wei Ding
Jan 25, 2011·Developmental and Comparative Immunology·Yi-Hong ChenJian-Guo He
Sep 14, 2011·Annual Review of Entomology·Gonzalo Giribet, Gregory D Edgecombe
Feb 23, 2012·Scientific Reports·Jesus Lozano, Xavier Belles
Citations
Apr 16, 2013·Fish & Shellfish Immunology·Xiaolan LiShigui Jiang
Oct 17, 2013·PloS One·Michelle L Flenniken, Raul Andino
Oct 16, 2016·Annual Review of Virology·Vanesa Mongelli, Maria-Carla Saleh
Jan 8, 2014·Peptides·Olivier ChristiaensGuy Smagghe
Oct 15, 2019·Annual Review of Entomology·Kun Yan Zhu, Subba Reddy Palli
Jul 30, 2016·Pest Management Science·Yu-Hsien LinHow-Jing Lee
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·June-Sun YoonSubba Reddy Palli
Jan 29, 2019·Frontiers in Physiology·Chao YeJin-Jun Wang
Aug 23, 2020·Insects·Ritesh G JainNeena Mitter
Mar 21, 2020·Frontiers in Immunology·Alessandro TorriMaria-Carla Saleh
Jan 9, 2021·Annual Review of Entomology·Bryony C Bonning, Maria-Carla Saleh
Jan 27, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Bi-Yue DingJin-Jun Wang
Dec 29, 2020·Journal of Insect Physiology·Anastasia M W CooperKun Yan Zhu
Apr 26, 2021·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·José Carlos MontañésJosé Luis Maestro