Super magnetic nanoparticles NiFe2O4, coated with aluminum-nickel oxide sol-gel lattices to safe, sensitive and selective purification of his-tagged proteins

Protein Expression and Purification
Seyede Zohreh Mirahmadi-ZareMohammad-Hossein Nasr-Esfahani

Abstract

Super magnetic nanoparticle NiFe2O4 with high magnetization, physical and chemical stability was introduced as a core particle which exhibits high thermal stability (>97%) during the harsh coating process. Instead of multi-stage process for coating, the magnetic nanoparticles was mineralized via one step coating by a cheap, safe, stable and recyclable alumina sol-gel lattice (from bohemite source) saturated by nickel ions. The TEM, SEM, VSM and XRD imaging and BET analysis confirmed the structural potential of NiFe2O4@NiAl2O4 core-shell magnetic nanoparticles for selective and sensitive purification of His-tagged protein, in one step. The functionality and validity of the nickel magnetic nanoparticles were attested by purification of three different bioactive His-tagged recombinant fusion proteins including hIGF-1, GM-CSF and bFGF. The bonding capacity of the nickel magnetics nanoparticles was studied by Bradford assay and was equal to 250 ± 84 μg Protein/mg MNP base on protein size. Since the metal ion leakage is the most toxicity source for purification by nickel magnetic nanoparticles, therefor the nickel leakage in purified final protein was determined by atomic absorption spectroscopy and biological activity of final purif...Continue Reading

References

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May 12, 2004·Protein Expression and Purification·Ryan J WilkinsonGeoffrey Francis
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Apr 7, 2012·Chemical Society Reviews·Miriam ColomboWolfgang J Parak

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Citations

Dec 19, 2016·International Journal of Biological Macromolecules·Belma Zengin KurtZehra Durmus
Feb 19, 2020·International Journal of Biological Macromolecules·Flávia F MagalhãesAna P M Tavares
Oct 24, 2017·ACS Applied Materials & Interfaces·Débora R LimaAlexandre Pancotti

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