Super-resolution microscopy via ptychographic structured modulation of a diffuser

Optics Letters
Pengming SongGuoan Zheng

Abstract

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the objective lens to modulate the complex light waves from the object. The otherwise inaccessible high-resolution object information can thus be encoded into the captured images. We then employ a ptychographic phase retrieval process to jointly recover the exit wavefront of the complex object and the unknown diffuser profile. Unlike the illumination-based super-resolution approach, the recovered image of our approach depends upon how the complex wavefront exits the sample-not enters it. Therefore, the sample thickness becomes irrelevant during reconstruction. After recovery, we can propagate the super-resolution complex wavefront to any position along the optical axis. We validate our approach using a resolution target, a quantitative phase target, a two-layer sample, and a thick polydimethylsiloxane sample. We demonstrate a 4.5-fold resolution gain over the diffraction limit. We also show that a four-fold resolution gain can be achieved with as few as ∼30 images. The reported approach may provi...Continue Reading

References

Jun 23, 2009·Ultramicroscopy·Andrew M Maiden, John M Rodenburg
Apr 12, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Andrew M MaidenJohn M Rodenburg
Jun 15, 2011·Physical Review Letters·E G van PuttenA P Mosk
Jul 30, 2011·Physical Review Letters·Youngwoon ChoiWonshik Choi
Nov 4, 2011·Optics Letters·Youngwoon ChoiWonshik Choi
Sep 23, 2014·Nature Photonics·Guoan ZhengChanghuei Yang
Sep 27, 2014·Physical Review Letters·Chunghyun ParkYongKeun Park
Sep 15, 2015·Optics Express·Richard M ClareFranz Pfeiffer
Jan 24, 2018·Biomedical Optics Express·Kaikai GuoGuoan Zheng
Nov 25, 2018·Optics Express·Samuel McDermott, Andrew Maiden

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Citations

Aug 28, 2020·Journal of Biophotonics·Zichao BianGuoan Zheng
Sep 30, 2020·Optics Express·Chengcheng ChangJianqiang Zhu
Nov 3, 2021·Lab on a Chip·Pengming SongGuoan Zheng

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