Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.

Chemical Science
Andrew T FrawleyHarry L Anderson

Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

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Citations

Nov 6, 2021·Nanoscale·Alan M SzalaiFernando D Stefani

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Methods Mentioned

BETA
super-resolution microscopy
FRET
confocal microscopy
Fluorescence
fluorescence correlation spectroscopy
FCS

Software Mentioned

MINFIELD
DyMIN
RESCue
RESOLFT

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